Protein changes in response to lead stress of lead-tolerant and lead-sensitive industrial hemp using SWATH technology
- Published
- Accepted
- Subject Areas
- Agricultural Science, Biochemistry, Plant Science
- Keywords
- Industrial hemp (Cannabis sativa L.), Pb stress, SWATH, Pb-stress adaption
- Copyright
- © 2019 Xia et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. Protein changes in response to lead stress of lead-tolerant and lead-sensitive industrial hemp using SWATH technology. PeerJ Preprints 7:e27527v1 https://doi.org/10.7287/peerj.preprints.27527v1
Abstract
Hemp is a Pb-tolerant and Pb-accumulating plant, and the study of its tolerance mechanisms could facilitate the breeding of hemp with enhanced Pb tolerance and accumulation. In the present study, we took advantage of SWATH (sequential window acquisition of all theoretical mass spectra) technology to study the difference in proteomics between the Pb-tolerant seed-type hemp variety Bamahuoma (BM) and the Pb-sensitive fiber-type hemp variety Yunma 1(Y1) under Pb stress (3g/kg soil). A total of 63 and 372 proteins differentially expressed under Pb stress relative to control conditions were identified with liquid chromatography electro spray ionization tandem mass spectrometry in BM and Y1, respectively; with each of these proteins being classified into 14 categories. Hemp adapted to Pb stress through accelerating ATP metabolism; enhancing respiration, light absorption and light energy transfer; promoting assimilation of intercellular nitrogen (N) and carbon (C); eliminating reactive oxygen species; regulating stomatal development and closure; improving exchange of water and CO2 in leaves; promoting intercellular transport; preventing aggregation of unfolded proteins; degrading misfolded proteins; and increasing the transmembrane transport of ATP in chloroplasts. Our results provide important reference protein and gene information for future molecular studies into the resistance and accumulation of Pb in hemp.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
List of hemp proteinsindentified in this study
Supplementary file 1 List of hemp proteinsindentified in this study. Three control samples of Y1 were labeled with tags YD1, YD2 and YD3, three Pb-treatment samples of Y1 were labeled with tags YC1, YC2 and YC3, three control samples of BM were labeled with tags BD1, BD2 and BD3, three Pb-treatment samples of BM were labeled with tags BC1, BC2 and BC3.
List of differentially expressed proteins in BM leave under Pb stress
List of differentially expressed proteins in BM leave under Pb stress (Control and Pb-stress samples were labeled with BD1,BD2,BD3 and BC1,BC2,BC3 tags, respectively)
List of differentially expressed proteins in Y1 leave under Pb stress
List of differentially expressed proteins in Y1 leave under Pb stress (Control and Pb-stress samples were labeled with YD1,YD2,YD3 and YC1,YC2,YC3 tags, respectively)