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Generating single cell-derived knockout clones in mammalian cells with CRISPR/Cas9

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RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
15 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
16 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
16 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
16 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
16 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
16 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
16 days ago
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
RT @JSheltzer: Our new paper/protocol – on how to make knockout clones in mammalian cells with CRISPR – is up at @PeerJPreprints: https://t…
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Additional Information

Competing Interests

The authors declare no competing interests.

Author Contributions

Christopher J Giuliano conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Ann Lin conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jason Sheltzer conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Funding

Research in the Sheltzer Lab is supported by an NIH Early Independence Award (1DP5OD021385), a Breast Cancer Alliance Young Investigator Award, a Damon Runyon- Rachleff Innovation Award, a Gates Foundation Innovative Technology Solutions grant, and a CSHL-Northwell Translational Cancer Research Grant. This work was performed with assistance from the CSHL Flow Cytometry Shared Resource, which is supported by the CSHL Cancer Center Support Grant 5P30CA045508. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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