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Supplemental Information

List of samples used in the study

Each sample is indicated by Species name, winter or spring type (for rapeseed), line id number from the catalog and id number used in mass specrometry analysis.

DOI: 10.7287/peerj.preprints.27501v1/supp-1

UPLC-MS data

TableA: UPLC-MS data for sunflower and rapeseed. For each sample in the table the information about species, method of analysis (UPLC-5 and UPLC-400), mass spectrometry id number and the intensity of each FA are presented. tableB: sunflower UPLC-5 statistics. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented. tableC: sunflower UPLC-400 statistics. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented. tableD: rapeseed UPLC-5 statistics. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented. tableE: rapeseed UPLC-400. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented. tableF: repeatability control: technical quality control samples (tQC). For each tQC the intensity of each FA are presented. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented calculated from tQC samples.

DOI: 10.7287/peerj.preprints.27501v1/supp-2

GC-FID data

TableA: GC-FID data for sunflower and rapeseed. For each sample in the table the information about species, line id and GC data for each FA are presented.tableB: rapeseed GC-FID statistics. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented. tableC: sunflower GC-FID statistics. For each FA mean value, standard deviation, minimum and maximum values and coefficient of variation are presented.

DOI: 10.7287/peerj.preprints.27501v1/supp-3

Example of isotopic pattern

A. Extracted ion chromatogram for 18:1 FA B. MS spectrum for 18:1 FA with 3 isotope peaks. X-axis -retention time in min, Y-axis -intensity in counts.

DOI: 10.7287/peerj.preprints.27501v1/supp-4

Variability between lines

The abundance of major FAs vary between sunflower samples measured by UPLC-5. Each colored line -chromatogram of 1 sample. X-axis -retention time in min, Y-axis -intensity in counts.

DOI: 10.7287/peerj.preprints.27501v1/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Alina Chernova conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Pavel Mazin analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Svetlana Goryunova analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Denis Goryunov authored or reviewed drafts of the paper.

Yakov Demurin conceived and designed the experiments.

Lyudmila Gorlova performed the experiments.

Anna Vanyushkina conceived and designed the experiments.

Waltraud Mair conceived and designed the experiments.

Nikolai Anikanov performed the experiments.

Ekaterina Yushina performed the experiments.

Anna Pavlova contributed reagents/materials/analysis tools.

Elena Martynova authored or reviewed drafts of the paper, approved the final draft.

Sergei Garkusha performed the experiments.

Zhanna Mukhina project administration.

Elena Savenko performed the experiments.

Philipp Khaitovich approved the final draft, supervision.

Data Deposition

The following information was supplied regarding data availability:

Figure S1: Example of isotopic pattern: A. Extracted ion chromatogram for 18:1 FA B. MS spectrum for 18:1 FA with 3 isotope peaks, Figure S2: Variability between lines. The abundance of major FAs vary between sunflower samples measured by UPLC-5, Table S1: List of samples used in the study, Table S2: GC-FID data. TableA: GC-FID data for sunflower and rapeseed, tableB: rapeseed GC-FID statistics, tableC: sunflower GC-FID statistics, Table S3: UPLC-MS data. TableA: UPLC-MS data for sunflower and rapeseed, tableB: sunflower UPLC-5 statistics, tableC: sunflower UPLC-400 statistics, tableD: rapeseed UPLC-5 statistics, tableE: repeseed UPLC-400.

The raw data are provided in the Supplemental Files.

Funding

This research was funded by the Ministry of Science and Higher Education of the Russian Federation (Grant No. 14.609.21.0099, Identification No. RFMEFI609l6X0099). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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