MicroRNA-331-3p affects preadipocytes’ proliferation and differentiation and fatty acid accumulation in Laiwu pigs
- Published
- Accepted
- Subject Areas
- Cell Biology, Genetics
- Keywords
- miR-331-3p, Yorkshire pig, dual luciferase analyze, gene expression, Laiwu pig
- Copyright
- © 2019 Chen et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2019. MicroRNA-331-3p affects preadipocytes’ proliferation and differentiation and fatty acid accumulation in Laiwu pigs. PeerJ Preprints 7:e27494v1 https://doi.org/10.7287/peerj.preprints.27494v1
Abstract
The proliferation and differentiation of preadipocytes are regulated by microRNAs (miRNAs), hormones and other factors. This study aimed to investigate the effects of miR-331-3p on the proliferationand differentiation of preadipocytes in addition to fatty acid metabolism. The data indicated that miR-331-3p is a novel regulator of cellular differentiation. It was observed that miR-331-3p was capable of inhibiting cellular proliferation. Furthermore, miR-331-3p was highly expressed during cellular differentiation andappeared to promote the process. In addition, dual fluorescein analysis showed that dihydrolipoamideS-succinyltransferase (DLST) is a target gene of miR-331-3p, and over-expression of miR-331-3p could regulate the metabolism of fatty acids in the citrate pyruvate cycle by targeting DLST expression. In summary, these findings indicated that miR-331-3p exerts contrasting effects on the processes ofproliferation and differentiation of preadipocytes.
Author Comment
This is a submission to PeerJ for review.