Integrating the extracellular, intracellular, and intercellular pathogenic processes of the microbiome through glucose saturation, inhibition of the acetyl-CoA carboxylase subunit accA with asRNA, and through quantifying cell-to-cell quorum sensing
- Published
- Accepted
- Subject Areas
- Genetics, Microbiology, Immunology, Infectious Diseases, Synthetic Biology
- Keywords
- Microbiome, Quorum Sensing, Pathogenesis, Antisense RNA, Lux-S Gene, Acetyl-CoA Carboxylase, qPCR, Plasmids, Glucose Absorption, Fermentation
- Copyright
- © 2018 Hillman
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Integrating the extracellular, intracellular, and intercellular pathogenic processes of the microbiome through glucose saturation, inhibition of the acetyl-CoA carboxylase subunit accA with asRNA, and through quantifying cell-to-cell quorum sensing. PeerJ Preprints 6:e27459v1 https://doi.org/10.7287/peerj.preprints.27459v1
Abstract
Bacteria ferment the glucose, from fiber, into Short Chain Fatty Acids, which help regulate many biochemical processes and pathways. We cultured Escherichia coli in Luria Broth enhanced with 15mM and 5mM of glucose. The 15mM concentration of qPCR products measured , for the target gene accA was 4,210 ng/μL. The 7.5μM sample had a concentration equaled to 375 ng/μL, and the 0μM sample had an accA concentration of 196 ng/μL. The gene accA, 1 of 4 subunits for the Acetyl-CoA Carboxylase enzyme, was suppressed by asRNA, producing a qPCR concentration of 63ng/μL. Antisense RNA for accA reduced the amount of Lux-S, a vital gene needed for propagating quorum-sensing signal molecules. Our purpose was to provide a more cumulative perspective for the pathogenesis of disease within the microbiome.
Author Comment
The purpose for our study was to construct a culmulative understanding of the processes 384 within the inner mechanisms of the intestinal microbiome. The microbiome is a vast organ, so our aim 385 was to attempt to compile a small part of its large navigational map to identify possible locales for future 386 synbiotic research and study.