Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine
- Published
- Accepted
- Subject Areas
- Agricultural Science, Developmental Biology
- Keywords
- MMPs, Apoptosis, TIMPs, Bovine, Luteal cell
- Copyright
- © 2018 Kim et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Expression of matrix metalloproteinases to induce the expression of genes associated with apoptosis during corpus luteum development in bovine. PeerJ Preprints 6:e27446v1 https://doi.org/10.7287/peerj.preprints.27446v1
Abstract
Here we investigated the expressions of apoptosis-associated genes known to induce programmed cell death through mRNA expressions of two matrix metalloproteinases (MMPs) that are involved in the degradation of collagen and basal membrane in luteal cells cultured in the treatment media. Our results show that the activity of MMP-2 gelatinase was higher in the CL2 and CL1 of luteal phase, was gradually decreased in the CH2 and CH3 of luteal phase. In particular, the expressions of P4-r and survival-associated genes (IGFr, PI3K, AKT, and mTOR) were strongly induced during CL3 stage, whereas the levels of these genes in CL were lower during CL2 and CL1 stages. And in the cultured lutein cell analyzed result, we found that as MMPs increase, genes related to apoptosis ( 20α-HSD and Casp-3) also increase. In other words, the results for P4-r and survival-related gene expression patterns in the luteal cells were contrary to the MMPs activation results. These results indicate that active MMPs are differentially expressed to induce the expression of genes associated with programmed cell death from the degrading luteal cells. Therefore, our results suggest that the MMPs activation may lead to luteal cell development or death.
Author Comment
This is a preprint submission to PeerJ Preprints.
Supplemental Information
Raw data exported from the ELISA of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 1
Raw data exported from the Real-Time PCR of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 2 and Fig. 3
Raw data exported from the Real-Time PCR applied for data analyses and preparation for the detailed investigation shown in Fig. 4, 5 and 6 for the time period of 24–96 h
Raw data exported from the zymography of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 3
Raw data exported from the Immunohistochemistry of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 3
Location and expression pattern analysis of MMP protein in corpus luteum stage, raw data for Fig 3A (Compression file #1).
Raw data exported from the Immunohistochemistry of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 3
Location and expression pattern analysis of MMP protein in corpus luteum stage, raw data for Fig 3A (Compression file #2).
Raw data exported from the Immunohistochemistry of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 3
Location and expression pattern analysis of MMP protein in corpus luteum stage, raw data for Fig 3A (Compression file #3).
Raw data exported from the Immunohistochemistry of corpus luteum applied for data analyses and preparation for the detailed investigation shown in Fig. 3
Location and expression pattern analysis of MMP protein in corpus luteum stage, raw data for Fig 3A (Compression file #4).