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Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars

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Song, Xu, et al: Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars https://t.co/KM544e4AJc
23 days ago
Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars https://t.co/qySZGCPK7D
Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars https://t.co/VvozqWTn4D MicroRNAs (miRNAs) play an important role in plant resistance to pathoge…
Identification and characterization of miRNA169 family members in banana (Musa acuminata L.) that respond to fusarium oxysporum f. sp. cubense infection in banana cultivars https://t.co/LVtWWDmK4h
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Supplemental Information

Additional Files1,3,4,6,7,8

Additional file 1: MiR169 family mature sequences of all crops

Additional file 3: The targets of ma-miR169 family

Additional file 4: KEGG Pathway of the targets

Additional file 6: The FPKM values expressed of HG and BX cultivars(Reads per Kilo bases per Million reads values)

Additional file 7: The primer pairs for miRNAs and targets comfirmation and quantitative detection

Additional file 8: Expression profiles of mature ma-miR169a, ma-miR169b and their targets in root in response to Fusarium wilt

DOI: 10.7287/peerj.preprints.27369v1/supp-1

The sequences of the miR169 family members

Evolutionary analysis clustered the miR169 family.

DOI: 10.7287/peerj.preprints.27369v1/supp-2

The pathways of target genes

The pathways of target genes. 1)Glycine, serine and threonine metabolism, 2)Pentose and glucuronate interconversions, 3)Glycerolipid metabolism, 4)Amino sugar and nucleotide sugar metabolism, 5)Starch and sucrose metabolism

DOI: 10.7287/peerj.preprints.27369v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Shun Song conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, approved the final draft.

Yi Xu performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, approved the final draft.

Dongmei Huang performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.

Muhammad Aleem Ashraf analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jingyang Li contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Wei Hu contributed reagents/materials/analysis tools, approved the final draft.

Zhiqiang Jin contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Changying Zeng conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Fenling Tang contributed reagents/materials/analysis tools, approved the final draft.

Biyu Xu contributed reagents/materials/analysis tools, approved the final draft.

Huicai Zeng contributed reagents/materials/analysis tools, approved the final draft.

Yujia Li contributed reagents/materials/analysis tools, approved the final draft.

Jianghui Xie conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

1. miRBase: the microRNA database.

http://www.mirbase.org/cgi-bin/mirna_summary.pl?fam=MIPF0000012

2. GSMUA_Achr8G16690_001: https://phytozome.jgi.doe.gov/pz/portal.html#!gene?search=1&detail=1&method=4987&searchText=transcriptid:32311987

GSMUA_Achr4G12800_001: https://phytozome.jgi.doe.gov/pz/portal.html#!gene?search=1&detail=1&method=4987&searchText=transcriptid:32297417

3. Designed primers for target genes in the Banana Genome Hub, http://banana-genome-hub.southgreen.fr/primer_designer, after got the RT reverse primer, RT forward primer, and PCR forward primer. After setting relevant parameters, all primers designed were verified experimentally, and then the ones with good specificity are available in Additional File 7.

Funding

This work was supported by the National Natural Science Foundation of China (NSFC, No. 31501371), the Commonwealth Industry (Agriculture) Specific Fund (201503110), the National Key Research and Development Program of China (2016YFD0200104) and the Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630092017011). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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