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Supplemental Information

Supplementary materials: Data files for effects of pollen on C. bombi

Dataset 1: Effects of extracts of individual pollens.

Dataset 2: Effects of individual and mixed pollen extracts.

Dataset 3: Effects of chemical additions.

"Row" and "column" refer to locations on the 96-well test plate

"conc" indicates the concentration in percent extract (or stock solution) by volume in the final sample

"net0" through "net24" are the net OD readings (difference in OD 630 nm between samples and cell-free controls at the corresponding concentration and timepoint) at each timepoint, with time given in hours.

"integral" is the estimated area under the curve of OD vs time, calculated by the "grofit" function in R

DOI: 10.7287/peerj.preprints.2730v1/supp-1

Supplementary Figures for Palmer-Young EC, Pollen extracts increase growth of a trypanosome parasite of bumble bees

Supplementary Figure 1. Growth curves for the individual pollen experiment.

Supplementary Figure 2. Growth curves for the mixed-pollen experiment

Supplemental figure 3. Growth curves for the chemical additions experiment.

DOI: 10.7287/peerj.preprints.2730v1/supp-2

Additional Information

Competing Interests

The authors declare that there are no competing interests.

Author Contributions

Evan C Palmer-Young conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary file.


This research was funded by the United States Department of Agriculture (USDA: Agricultural and Food Research Initiative (AFRI) Food, Agriculture, Natural Resources and Human Sciences Education and Literacy Initiative (ELI) Predoctoral Fellowship Award Number: 2016-67011-24698 to ECPY); and by the Garden Club of America ( (Centennial Pollinator Fellowship to ECPY). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the funding agencies. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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