Genetic diversity and population structure of Chinese jujube (Ziziphus jujuba Mill.) and sour jujube (Ziziphus acidojujuba Mill.) using inter-simple sequence repeat (ISSR) Markers
Author and article information
Abstract
The Chinese jujube (Ziziphus jujuba Mill.) originates from sour jujube (Ziziphus acidojujuba Mill.) and is an economically important genus in the Rhamnaceae family. However, little is known about the genetic relationship between jujube cultivars and wild species. In this study, we estimated the genetic variation and relationships between 85 jujube cultivars and 55 sour jujube individuals by ISSR markers. Of 216 ISSR primers, 110 were able produce amplified product(s) and 28 showed polymorphisms, accounting for 50.9% and 25.5% of total primers respectively. A total of 89 loci were amplified with 28 primers, of which 42 loci (47.2%) were polymorphic, and most of primers exhibited highly PIC values. Cluster analysis and population structure analysis roughly divided the 140 accessions into two major groups. One group included all jujube cultivars and some sour jujube individuals, and the other group included remaining sour jujube individuals. Most jujube cultivars have a certain correlation with their origin, and there are obvious gene exchanges between sour jujube and jujube cultivars. The results provide a useful basis for jujube germplasm conservation, genetic improvement and evolution research.
Cite this as
2018. Genetic diversity and population structure of Chinese jujube (Ziziphus jujuba Mill.) and sour jujube (Ziziphus acidojujuba Mill.) using inter-simple sequence repeat (ISSR) Markers. PeerJ Preprints 6:e27088v1 https://doi.org/10.7287/peerj.preprints.27088v1Author comment
This is a submission to PeerJ for review.
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Supplemental Information
Inferred ancestry of the 140 accessions based on Bayesian analysis
Distribution of Q-value of 140 accessions in two groups by model-based cluster method
Summary of genetic variation statistics for 28 ISSR primers from 85 jujube cultivars and 55 sour jujube individuals
Distribution of Q-value of 85 jujube cultivars and 55 sour jujube individuals by model-based cluster method
Additional Information
Competing Interests
The authors declare that they have no competing interests.
Author Contributions
Shipeng Li conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, approved the final draft.
Mingxin Guo performed the experiments.
Pengcheng Fu analyzed the data, prepared figures and/or tables.
Hongxia Liu performed the experiments.
Xusheng Zhao conceived and designed the experiments, authored or reviewed drafts of the paper, approved the final draft.
DNA Deposition
The following information was supplied regarding the deposition of DNA sequences:
The primers used in this study has been uploaded as Supplemental Table 2.
Data Deposition
The following information was supplied regarding data availability:
After the paper is accepted, the raw data generated in this study will be deposited in figshare (https://figshare.com/).
Funding
This work was supported by the Natural Science Foundation of China (NO. 31600296), Natural Science Foundation of Henan Province (NO. 162300410198) and Science and Technology Open Cooperation Project of Henan Province (NO. 172106000049). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.