Starch branching enzymes (SBEs) in banana: genome-wide identification and expression analysis reveal their involvement in fruit development, ripening and regulated responses to abiotic/biotic stresses
- Published
- Accepted
- Subject Areas
- Agricultural Science, Biotechnology, Molecular Biology, Plant Science
- Keywords
- Banana, Starch branching enzyme, Genome-wide characteristics, Fruit development, Biotic stress, Abiotic stress
- Copyright
- © 2018 Miao et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Starch branching enzymes (SBEs) in banana: genome-wide identification and expression analysis reveal their involvement in fruit development, ripening and regulated responses to abiotic/biotic stresses. PeerJ Preprints 6:e27045v1 https://doi.org/10.7287/peerj.preprints.27045v1
Abstract
Starch branching enzyme (SBE), which is one of the key enzymes associated with amylopectin biosynthesis, plays important roles in variable biological processes. Despite its importance, SBE is rarely studied in the banana (Musa acuminata L.) which is a typical starchy fruit. Here, a family of ten SBE proteins (MaSBE) was firstly identified through genome-wide characterization in M. acuminata, which could be clustered into three subfamilies. Systematic transcriptome analysis revealed temporal and spatial expression variations of MaSBE genes and differential response patterns under abiotic and biotic stresses in both banana genotypes, Fen Jiao (FJ) and BaXi Jiao (BX). Moreover, MaSBE2.4 was temporally regulated during fruit development and ripening as well as in response to various abiotic/biotic stresses in both genotypes. Specifically, MaSBE2.3 expression level was higher in FJ than in BX following cold, salt, and drought stress treatments, and it was specifically induced by fungal infection in BX. Characterization of hormone- and stress-related cis-acting elements in the promoters of MaSBE genes suggests their multiple biological functions. In conclusion, our study provides new insights into the complex transcriptional characteristics of the SBE genes, and demonstrates their crucial roles in improving amylopectin biosynthesis and strengthening stress resistance in banana.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Table S1. Characteristics of SBE proteins in banana
Table S2. The primer sequences used for qRT-PCR
Table S3. Conserved amino acid motifs and functional annotation of banana MaSBE proteins
Table S4. Expression data of MaSBE genes in different tissues of BX and FJ bananas
Asterisks indicate significant difference between the BX and FJ (*p<0.05; **p<0.01).
Table S5. Expression data of MaSBE genes in different stages of fruit development and ripening in BX and FJ bananas
Asterisks indicate significant difference between the BX and FJ (*p<0.05; **p<0.01).
Table S6. Expression data of MaSBE genes after various abiotic stress treatments in BX and FJ bananas
Table S7. Expression data of MaSBE genes after fungal infection in BX and FJ bananas
Asterisks indicate significant difference between the BX and FJ (*p<0.05; **p<0.01).