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Ocean acidification alters morphology of all otolith types in Clark’s anemonefish (Amphiprion clarkii)

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396 days ago
RT @EnvSchool: Hannigan, @TlustyM, @AndrewLRhyne Group (PhD student Rob Holmberg lead author) preprint has been published today in @PeerJPr…
RT @EnvSchool: Hannigan, @TlustyM, @AndrewLRhyne Group (PhD student Rob Holmberg lead author) preprint has been published today in @PeerJPr…
RT @EnvSchool: Hannigan, @TlustyM, @AndrewLRhyne Group (PhD student Rob Holmberg lead author) preprint has been published today in @PeerJPr…
RT @EnvSchool: Hannigan, @TlustyM, @AndrewLRhyne Group (PhD student Rob Holmberg lead author) preprint has been published today in @PeerJPr…
RT @EnvSchool: Hannigan, @TlustyM, @AndrewLRhyne Group (PhD student Rob Holmberg lead author) preprint has been published today in @PeerJPr…
Hannigan, @TlustyM, @AndrewLRhyne Group (PhD student Rob Holmberg lead author) preprint has been published today in @PeerJPreprints https://t.co/XUm26tL7Cz #Aquaculture,FisheriesandFishScience #MarineBiology
397 days ago
Ocean acidification alters morphology of all otolith types in Clark’s anemonefish (Amphiprion clarkii) https://t.co/842M4nDDXH
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Supplemental Information

Tank Means for Statistical Analysis

Includes SL (mm); Area/SL, Perimeter/SL (um/mm); circularity (dimensionless); lateral development (scale of 1-5); percent visible crystals (scale of 5-50%); as well as tank counts of stocking density, settled, unsettled, dead (mort), and surviving (remaining) fish at the end of the experimental trial.

DOI: 10.7287/peerj.preprints.27040v1/supp-1

Statistical Analyses

Annotated R code used to run statistical analyses on fish and otolith morphometric data.

DOI: 10.7287/peerj.preprints.27040v1/supp-2

Micrograph Scoring Rubric

Rubric used for training and guiding scanning electron micrograph readers through scoring of four otolith mineralogical metrics. NOTE: “Core development” in the rubric has been renamed “lateral development” in the manuscript. In the rubric, “core” refers not to the otolith’s core but to the center of its lateral face.

DOI: 10.7287/peerj.preprints.27040v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Robert Holmberg analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Eric Wilcox-Freeburg conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Andrew L Rhyne conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Michael F Tlusty conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Alan Stebbins analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Steven W Nye Jr. analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Aaron Honig analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Amy E Johnston analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Christine M San Antonio analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Bradford Bourque conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Robyn E Hannigan conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

IACUC at Roger Williams University in Bristol, Rhode Island provided full approval for this research.

Data Deposition

The following information was supplied regarding data availability:

PeerJ Supplemental Material;

BCO-DMO (OA-OTO MIN; https://www.bco-dmo.org/project/2240);

GitHub https://github.com/edfreeburg

Funding

This study was supported by grants from the National Science Foundation [NSF-CRI-OA-1220480 (PIs: Hannigan, Tlusty, Rhyne)] and the National Science Foundation Rhode Island Experimental Program to Stimulate Competitive Research [EPSCoR-RI (PI: Rhyne)]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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