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Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites

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Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites https://t.co/efB3PEXZyp https://t.co/sK17twdDNM | mis libros: https://t.co/KuP0dfu086
247 days ago
Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites https://t.co/P8eiXveYVP
peerJ: Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites https://t.co/EtkbIYVpU8
Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites https://t.co/efB3PEXZyp https://t.co/sK17twdDNM | mis libros: https://t.co/KuP0dfu086
337 days ago
Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites https://t.co/Dc8NR2LObf
peerJ: Low genetic diversity in captive populations of the critically endangered Blue-crowned Laughingthrush (Garrulax courtoisi) revealed by a panel of novel microsatellites https://t.co/U3Vq5yIeMt
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Supplemental Information

Sample voucher numbers and sequence GenBank accession numbers of two captive Blue-crowned Laughingthrush (Garrulax courtoisi) populations used in this article. (OPHK= Ocean Park, Hong Kong, SYS=Sun Yat-sen University)

DOI: 10.7287/peerj.preprints.27038v2/supp-1

Pairwise genetic relatedness among analysed pairs of Blue-crowned Laughingthrush individuals

Genetic kinship between each pair of individuals that has the highest likelihood at lower diagonal (PO = Parent / Offspring; FS = Full siblings; HS = Half siblings; U = Unrelated), and the pairwise relatedness based on the Queller and Goodnight estimator (RQG) are provided for each pair of individuals at upper diagonal. Table S2a. indicate OPHK population of Blue-crowned Laughingthrush, and Table S2b. indicate NCZ population of Blue-crowned Laughingthrush. (OPHK: Ocean Park Hong Kong, NCZ: Nanchang Zoo).

DOI: 10.7287/peerj.preprints.27038v2/supp-2

Raw Data 2. Genotype of the 12 microsatellite loci in a sample set of two captive Blue-crowned Laughingthrush populations

Raw Data. Abbreviations indicate different Blue-crowned Laughingthrush populations (OPHK: Ocean Park Hong Kong, NCZ: Nanchang Zoo).

DOI: 10.7287/peerj.preprints.27038v2/supp-3

Analysis of statistical power to detect significant differentiation based on the 12 microsatellite markers used in this study

Datasets with eight predefined levels of population differentiation (FST values of 0.001, 0.0025, 0.005, 0.01, 0.02, 0.05, 0.075) were generated using POWSIM. Statistical power was defined as the proportion of times the null hypothesis of equal allele frequencies across populations was rejected using a chi-square test or a Fisher’s exact test.

DOI: 10.7287/peerj.preprints.27038v2/supp-4

Raw data1. DNA sequences of two captive Blue-crowned Laughingthrush (Garrulax courtoisi) populations used in this article

DOI: 10.7287/peerj.preprints.27038v2/supp-5

Additional Information

Competing Interests

Guoling Chen is a master student and Yang Liu is an Associate Professor in Sun Yat-sen University. Chenqing Zheng and Xu Yang are bioinformaticians employed by Shenzhen Realomics Biological Technology Ltd. Nelson Wan is employed by Ocean Park Hong Kong Corporation. Daoqiang Liu is employed by Nanchang Zoo. Vivian Wing Kan, Fu and Yat-tung Yu are conservationists in The Hong Kong Bird Watching Society, a bird conservation NGO.

The authors declare that they have no competing interests.

Author Contributions

Guoling Chen performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper.

Chenqing Zheng analyzed the data, contributed reagents/materials/analysis tools.

Nelson Wan conceived and designed the experiments, performed the experiments, sampling.

Daoqiang Liu performed the experiments, sampling.

Vivian Wing Kan Fu conceived and designed the experiments.

Xu Yang contributed reagents/materials/analysis tools.

Yat-tung Yu conceived and designed the experiments.

Yang Liu analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All the animal operations were approved by the Institutional Ethical Committee of Animal Experimentation of Sun Yat-sen University (2005DKA21403-JK) and strictly complied with the ethical conditions by the Chinese Animal Welfare Act (20090606).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank Accession No. MH423582–MH423604

Data Deposition

The following information was supplied regarding data availability:

The raw data of microsatellite genotype are provided in supplementary files (Please see the Table S3).

The raw data/code is included in the manuscript (please see the line 322-323 and Table S1).

Funding

This work was supported by a grant from the Ocean Park Conservation Foundation, Hong Kong, China (No. BD03_1617) to Yang Liu. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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