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Elevated end systolic function in a rat model of temporal lobe epilepsy

, , , , , ,
1 A.I.Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland
2 Department of Psychology, University of Jyväskylä, Jyväskylä, Finland
3 Department of Mathematics, Le Moyne College, Syracuse, United States
4 Research Unit of Medical Imaging, University of Oulu, Oulu, Finland
5 Department of Diagnostic Radiology, University Hospital of Oulu, Oulu, Finland
6 Department of Biological Sciences, Le Moyne College, Syracuse, United States
DOI
10.7287/peerj.preprints.26999v1
Published
Accepted
Subject Areas
Neuroscience, Anatomy and Physiology
Keywords
cMRI, cardiac, rodent, epilepsy
Copyright
© 2018 Pirttimäki et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Pirttimäki T, Laakso H, Sierra A, Cunningham C, Liimatainen T, Gröhn O, DeRuisseau L. 2018. Elevated end systolic function in a rat model of temporal lobe epilepsy. PeerJ Preprints 6:e26999v1

Abstract

Sudden unexpected death in epilepsy (SUDEP) is a common cause of premature death amongst epilepsy patients. It is hypothesized to result from cardiorespiratory dysfunction, but the exact aetiology is unknown. The aim of this study was to determine if functional cardiovascular alterations were present in rats with chronic epileptic behaviour. Naive control rats were compared to a rat model of temporal lobe epilepsy that was induced using a repeated low-dose kainic acid (KA) protocol. The results indicate that end-systolic volume was significantly (p=0.01) higher in the epileptic group whilst end-diastolic volume did not reach significance (p=0.08). Ejection fraction, stroke volume, cardiac output, heart rate, body weight and heart size were also measured and appeared similar between groups. These initial data support the use cardiac magnetic resonance imaging (cMRI) to investigate cardiovascular changes across disease development of epilepsy-like behaviour, which may offer insight into understanding SUDEP.

Author Comment

This is a submission to PeerJ for review.

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Tiina Pirttimäki conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Hanne Laakso performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Alejandra Sierra contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Caitlin Cunningham contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, statistical analysis.

Timo Liimatainen contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Olli Gröhn conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Lara DeRuisseau conceived and designed the experiments, performed the experiments, analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All animal procedures were performed in accordance with the University of Eastern Finland animal care committee's regulations and in accordance with the guidelines of the European Community Council Directives 2010/63/EU.

Data Deposition

The following information was supplied regarding data availability:

Open Science Framework

Elevated end systolic function in a rat model of temporal lobe epilepsy

https://osf.io/2fp8v/?view_only=470403669f7a4d7799ce3847f06b86b0

Funding

This work was supported by the Finland Fulbright Commission, the Academy of Finland, FP7-HEALTH project 602102 (EPITARGET), Finnish Cultural Foundation and Suomen Aivosäätiö. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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