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Agrichemicals boost the effects of antibiotics on antibiotic resistance evolution

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RT @Jack_Heinemann: @barnoolut @Olivefarmer @GreenEpidemic @Agrivetbusiness @zinjabil @Botanygeek I think these are the papers @Olivefarmer…
85 days ago
RT @Jack_Heinemann: @barnoolut @Olivefarmer @GreenEpidemic @Agrivetbusiness @zinjabil @Botanygeek I think these are the papers @Olivefarmer…
@barnoolut @Olivefarmer @GreenEpidemic @Agrivetbusiness @zinjabil @Botanygeek I think these are the papers @Olivefarmer is referring to https://t.co/gxPD6Ef0M3 https://t.co/RFFHDxUxsS https://t.co/nbeeiUOUyw https://t.co/bFIlKQjOkb
402 days ago
Agrichemicals boost the effects of antibiotics on antibiotic resistance evolution https://t.co/vgscHkYjaR
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Supplemental Information

Growth curves of selectively resistant strains of E. coli in monoculture with or without herbicide, antibiotic, or both

A, C: determination of the growth rate r of Strhigh/low (AH204/AH211) and Tethigh/low (AH201/AH214), respectively. The gray line represents log transformed OD600 values; the dotted black line represents our estimate of growth during the exponential phase calculated using log(OD) values between 48 and 150 min, the period between the grey dashed vertical lines. In this period log(OD) increased approximately linearly with time indicating exponential growth. B, D: determination of the carrying capacity k of Strhigh/low (AH204/AH211) and Tethigh/low (AH201/AH214), respectively. The gray line represents OD600 values; the dotted black line represents the estimated logistic curve calculated by fitting a logistic growth model using non-linear least squares. Antibiotics were used 0.25µg/mL for Str and 0.05µg/mL for Tet; herbicide concentrations were 1830 ppm ae Kamba and 311 ppm ae Roundup.

DOI: 10.7287/peerj.preprints.26925v1/supp-1

Raw data suppl growth curvers tetracycline and roundup

DOI: 10.7287/peerj.preprints.26925v1/supp-5

Raw data suppl growth curves streptomycin and kamba

DOI: 10.7287/peerj.preprints.26925v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Brigitta Kurenbach performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Amy M Hill performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

William Godsoe analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.

Sophie van Hamelsveld performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, approved the final draft.

Jack A Heinemann conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

Raw data is provided in the form of spreadsheets.

Funding

AMH received the New Zealand Federation of Graduate Women and UC for support. This project received funding from the Brian Mason Trust (JAH) and donations to the UC Foundation (JAH) including from, inter alia, donors Third World Network (Malaysia) and the Sustainable Food Trust (UK). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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