Figure S1: Plots of length variation for six additional primers
Plot of primer binding sites and bar plots depicting length of utilised primers for the 10 most abundant OTUs in mock sample B (sequence data from Elbrecht & Leese 2017). The presence and read abundance for different taxa can vary based on the primer set used, thus while amplifying the same mock samples, the order of and taxa the 10 most abundant specimens is not identical between plots as OTUs are sorted by read abundance. The sample was amplified with the P5_BF1_0 + P7_BR1_4 and P5_BF2_0 + P7_BR1_4 primer set, and the length distribution of the incorporated primers is shown for the BR1 primer (A and B). The percentage of amplicons that incorporated a particular length are shown above each bar. Additional plots for further primer combinations on the next 2 pages (C, D, E).