Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants
- Published
- Accepted
- Subject Areas
- Agricultural Science, Plant Science
- Keywords
- bud multiplication, direct organogenesis, Sedum morganianum, Pachyveria pachytoides
- Copyright
- © 2017 Xu et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2017. Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants. PeerJ Preprints 5:e2656v2 https://doi.org/10.7287/peerj.preprints.2656v2
Abstract
This study aimed to establish a simple and efficient in vivo multiplication protocol by leaf cutting to satisfy the supply of young succulent ornamentals Pachyveria pachytoides and Sedum morganianum. The regenerability of leaves injected with 6-benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) in vivo were tested with common leaf cutting as control. Results showed a 100% shoot induction frequency using hormone-injeceted methods for the two species. The number of shoots per leaf of 4.0 or 6.0 mg l −1 BAP and 0.1 mg l −1 NAA injected in vivo (5.08-5.14 in P. pachytoides, and 6.22-6.74 for S. morganianum) were significantly greater than that of the other treatments. Since the h ormone-injected leaf cutting needs no aseptic operation which is necessary for in vitro multiplication, it is simple for the commercial production of the two species. The new in vivo propagation method would be of great interest for growers and breeders of succulent plants.
Author Comment
Our study established an efficient in vivo regeneration protocol by plant growth regulators injected leaves to meet the supply of young succulent ornamental plants of Pachyveria pachytoides. We believe the paper may be of particular interest to the readers of your journal as it provided a cheap in vivo propagation method, which would be useful for commercial production and breeding of succulent ornamental plants.