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Conservation of Symbiodinium spp. clade in the coral Pocillopora damicornis during the 2014 mass-bleaching event

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246 days ago
Conservation of Symbiodinium spp. clade in the coral Pocillopora damicornis during the 2014 mass-bleaching event https://t.co/ld8sA1Yq3G
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Supplemental Information

Supplemental figure 1. Agarose gel electrophoresis of DNA isolated from Symbiodinium associated with sampled P. damicornis colonies

Upper gel lanes 3-6 represent pre-bleached samples colonies 1, 2, 5, 6, respectively; upper gel lanes 7-10 represent bleached samples colonies 1, 2, 5, 6, respectively; and lower gel lanes 3-6 represent post-bleached samples colonies 1, 2, 5, 6, respectively (primers ss3z, ss5z; GelGreen stain, 1 kB Plus DNA Ladder (Invitrogen)).

DOI: 10.7287/peerj.preprints.26514v1/supp-1

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

James WA Murphy conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Narrissa P Spies analyzed the data, authored or reviewed drafts of the paper, approved the final draft.

Robert H Richmond conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Department of Land and Natural Resources - Division of Aquatic Resources coral collection permit 2015-06 and 2015-17 (O‘ahu, HI, USA).

Data Deposition

The following information was supplied regarding data availability:

The research in this article did not generate any data or code. These data sought to visualize patterns in closed profiles, and as such, only visual data was generated.

Funding

This project was financially supported through National Oceanic and Atmospheric Administration (grant number NA09NOS4780178) and the Charles H. and Margaret B. Edmondson Research Fund (University of Hawai‘i at Mānoa Department of Biology). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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