Ca2+ dynamics in zebrafish hyper-dorsalization of congenital anomaly
- Published
- Accepted
- Subject Areas
- Developmental Biology, Molecular Biology
- Keywords
- Yellow cameleon, Intracellular calcium, Zebrafish, Congenital anomaly
- Copyright
- © 2016 Tsuruwaka et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2016. Ca2+ dynamics in zebrafish hyper-dorsalization of congenital anomaly. PeerJ Preprints 4:e2644v1 https://doi.org/10.7287/peerj.preprints.2644v1
Abstract
Intracellular calcium ion (Ca2+) signaling is deeply involved in development, as demonstrated by the use of various Ca2+ sensors. Ca2+ patterns in wildtype and mutant zebrafish have been reported to date, however, not many zebrafish embryos with congenital anomaly have been studied yet. In the present study, we monitored serial Ca2+ levels of zebrafish embryos which showed inborn hyper-dorsalization using Ca2+ sensor, yellow cameleon, YC2.12. Our results showed clearly elevated Ca2+ levels in the presumptive dorsal part during gastrula period and in the dorsal region during segmentation period. The results suggested that monitoring Ca2+ levels may help to anticipate the region which influences a fate determination prior to morphogenesis.
Author Comment
We have recently reported Ca2+ dynamics of normal zebrafish development in PeerJ. Since our zebrafish research project will end shortly, we would like to report the valid data we have obtained so far.