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Supplemental Information

Analysis of mapping frequency of artificially-fragmented reads corresponding to the genomes the “Ca. Desulfopertinax cowenii” and “Ca. Desulforudis audaxviator”

Comparison of mapping frequency of artificially-fragmented 150 bp reads corresponding to the genomes the “Ca. Desulfopertinax cowenii” and “Ca. Desulforudis audaxviator” mapped to the opposite genome using a range of nucleotide similarity scores. Inset plot shows details between mapping similarity score of 95-100% and revealed a mapping similarity score of 96% restricted spurious matches to a frequency of 1%.

DOI: 10.7287/peerj.preprints.2592v1/supp-1

Phylogenetic analysis of “Ca. Desulfopertinax cowenii”, “Ca. Desulforudis audaxviator” and other closely related dsrA genes

Phylogenetic relationships between “Ca. Desulfopertinax cowenii”, “Ca. Desulforudis audaxviator”, and closely related dsrA genes. Black (100%), gray (≥80%), and white (≥50%) circles indicate nodes with bootstrap support, from 2000 replicates. The scale bar corresponds to 0.05 substitutions per nucleotide position.

DOI: 10.7287/peerj.preprints.2592v1/supp-2

Summary of metagenome sequence reads mapped to “Ca. Desulforudis audaxviator”, “Ca. D. audaxviator”-related scaffolds from IMG-M, and genomic bin A32 from metagenome U1362A

DOI: 10.7287/peerj.preprints.2592v1/supp-3

Metagenomes from the IMG database used in Figure 7A

DOI: 10.7287/peerj.preprints.2592v1/supp-4

Metagenomes with accessible raw reads from IMG, MG-RAST, and NCBI databases used in Figure 7B

DOI: 10.7287/peerj.preprints.2592v1/supp-5

Genomic bin purification using ProDeGe

DOI: 10.7287/peerj.preprints.2592v1/supp-6

Similarities in “Ca. Desulforudis audaxviator” and “Ca. D. audaxviator” by COG category with example genes

DOI: 10.7287/peerj.preprints.2592v1/supp-7

Genes in “Ca. D. cowenii” without homologs in “Ca. D. audaxviator” genome

DOI: 10.7287/peerj.preprints.2592v1/supp-8

Genes in “Ca. D. audaxviator” without homologs in “Ca. D. cowenii” genome

DOI: 10.7287/peerj.preprints.2592v1/supp-9

Ca. Desulfopertinax cowenii” genome in GenBank format

DOI: 10.7287/peerj.preprints.2592v1/supp-10

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Sean P Jungbluth conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Tijana Glavina del Rio contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Susannah G Tringe contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Ramunas Stepanauskas contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Michael S Rappé conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The genome sequences described here are accessible via IMG Genome ID 2615840622.

Data Deposition

The following information was supplied regarding data availability:

The raw sequence data used for this analysis are accessible via SRA accession numbers SRR3723048 (U1362A) and SRR3732688 (U1362B).

Funding

This research was supported by funding from National Science Foundation grants MCB06-04014 and OCE-1260723 (to MSR) and OCE-1136488 (to RS), the Center for Dark Energy Biosphere Investigations, a National Science Foundation-funded Science and Technology Center of Excellence (NSF award OCE-0939564), and from Department of Energy Joint Genome Institute Community Sequencing Award 987 (to RS). The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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