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Supplemental Information

Sequences of multiple sequence alignment of selected metazoan homologues of MED28 with F28F8.5a

Sequences in FastA format for direct submission to Promals.

DOI: 10.7287/peerj.preprints.2530v2/supp-1

Expression of F28F8.5a/b::GFP from a transgene including internal promoter and present in transgenic animals as extrachromosomal arrays

Panels A, C, E and G show images in Nomarski optics and corresponding panels B, D, F and H in GFP fluorescence. In the twofold stage (panels A and B, C and D), the transgene is expressed in epidermal cells - seam cells (panels B and D). The transgene is localized both in the cytoplasm and nuclei (panel B, arrowheads) or is found accented in nuclei (panel D, arrowheads, contrast and brightness were increased +20, original picture is provided as Raw Data). In the threefold embryo (panels E and F), the transgene is progressively localized in nuclei as well as in the cytoplasm of epidermal cells, arrowheads. Panels G and H show a complete L1 larva before hatching with the expression of the transgene predominantly in the cytoplasm of intestinal cells (arrow). Panels L, M and N show an adult hermaphrodite animal with F28F8.5::GFP fluorescence in nuclei of enterocytes and in the excretory cell and its channels (arrows). Panels O to Q show a proximal part of the body of a hermaphrodite L2 stage in confocal microscopy (panels P and Q that are parallel optical planes) and overview image in Nomarski optics (panel O). Arrows indicate excretory channels and the body of the excretory cell (lower arrow in panel Q). Arrowheads indicate nuclei of enterocytes with accumulated F28F8.5::GFP in a nuclear compartment surrounding the large nucleoli. F28F8.5::GFP is also localized diffusely in the cytoplasm of enterocytes. Bars represent 10 µm.

DOI: 10.7287/peerj.preprints.2530v2/supp-2

Raw images for Fig.2

Unprocessed images that were used for preparation of Fig.2

DOI: 10.7287/peerj.preprints.2530v2/supp-4

Additional Information

Competing Interests

Marta Kostrouchova is an academic editor at PeerJ.

No other competing interests declared.

Author Contributions

Markéta Kostrouchová conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

David Kostrouch conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Ahmed A Chughtai conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Filip Kaššák conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jan P. Novotný conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Veronika Kostrouchová performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Aleš Benda conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Michael W. Krause conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Vladimír Saudek conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Marta Kostrouchová conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, contributed with personal funds..

Zdenek Kostrouch conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, contributed with personal funds..

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary file.

Funding

This work was supported by the European Regional Development Fund “BIOCEV — Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University in Vestec” (CZ.1.05/1.1.00/02.0109) (The Start-Up Grant to the group Structure and Function of Cells in Their Normal State and in Pathology – Integrative Biology and Pathology (5.1.10)) and the LQ1604 National Sustainability Program II (Project BIOCEV-FAR) and the project Biocev (CZ.1.05/1.1.00/02.0109) from the Ministry of Education, Youth and Sports of Czech Republic; The grant PRVOUK-P27 from Charles University in Prague; The grant SVV260257/2016, SVV260149/2015 and SVV 260023/2014 from the Charles University in Prague. This work was supported by the project OPPK No. CZ.2.16/3.1.00/24024, awarded by European Fund for Regional Development (Prague & EU – We invest for your future). ZK and MK contributed with personal funds to this work. The funders (except authors) had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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