Sequences of multiple sequence alignment of selected metazoan homologues of MED28 with F28F8.5a
Sequences in FastA format for direct submission to Promals.
Expression of F28F8.5a/b::GFP from a transgene including internal promoter and present in transgenic animals as extrachromosomal arrays
Panels A, C, E and G show images in Nomarski optics and corresponding panels B, D, F and H in GFP fluorescence. In the twofold stage (panels A and B, C and D), the transgene is expressed in epidermal cells - seam cells (panels B and D). The transgene is localized both in the cytoplasm and nuclei (panel B, arrowheads) or is found accented in nuclei (panel D, arrowheads, contrast and brightness were increased +20, original picture is provided as Raw Data). In the threefold embryo (panels E and F), the transgene is progressively localized in nuclei as well as in the cytoplasm of epidermal cells, arrowheads. Panels G and H show a complete L1 larva before hatching with the expression of the transgene predominantly in the cytoplasm of intestinal cells (arrow). Panels L, M and N show an adult hermaphrodite animal with F28F8.5::GFP fluorescence in nuclei of enterocytes and in the excretory cell and its channels (arrows). Panels O to Q show a proximal part of the body of a hermaphrodite L2 stage in confocal microscopy (panels P and Q that are parallel optical planes) and overview image in Nomarski optics (panel O). Arrows indicate excretory channels and the body of the excretory cell (lower arrow in panel Q). Arrowheads indicate nuclei of enterocytes with accumulated F28F8.5::GFP in a nuclear compartment surrounding the large nucleoli. F28F8.5::GFP is also localized diffusely in the cytoplasm of enterocytes. Bars represent 10 µm.