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We present the first record of the orb-weaver spider Bertrana striolata from Amazonian lowlands (east of the Andes) in Ecuador, based on a specimen collected at the Tiputini Biodiversity Station, province of Orellana, Ecuador. In Ecuador, B. striolata was previously known from a single locality on the Pacific lowlands (4 km NE of the town of Pedro Vicente Maldonado, 550 m, province of Pichincha). To the best of our knowledge, this record also corresponds to the second locality of the species in the country, and the first record for the province of Orellana.
This manuscript contains the first record of a spider from Amazonian Ecuador. A version of this manuscript was submitted to a journal. However, this manuscript contains complete information about the record, and it will serve as a reference for studies on spider diversity in the Neotropics.