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YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance

, , , , , , ,
1 Department of Plant Biology, University of California, Davis, Davis, CA, United States
2 Natural Sciences Department, Castleton University, Castleton, Vermont, United States
3 Amaryllis Nucleics, Berkeley, CA, United States
DOI
10.7287/peerj.preprints.2211v2
Published
Accepted
Subject Areas
Developmental Biology, Genetics, Plant Science
Keywords
auxin, shade avoidance, phytochrome, photomorphogenesis
Copyright
© 2016 Müller-Moulé et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Müller-Moulé P, Nozue K, Pytlak ML, Palmer CM, Covington MF, Wallace AD, Harmer SL, Maloof JN. 2016. YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance. PeerJ Preprints 4:e2211v2

Abstract

Plants respond to neighbor shade by increasing stem and petiole elongation. Shade, sensed by phytochrome photoreceptors, causes stabilization of PHYTOCHROME INTERACTING FACTOR proteins and subsequent induction of YUCCA auxin biosynthetic genes. To investigate the role of YUCCA genes in phytochrome-mediated elongation we examined auxin signaling kinetics after an end-of-day far-red (EOD-FR) light treatment, and found that an auxin responsive reporter is rapidly induced within 2 hours of far-red exposure. YUCCA2, 5, 8, and 9 are all induced with similar kinetics suggesting that they could act redundantly to control shade-mediated elongation. To test this hypothesis we constructed a yucca2,5,8,9 quadruple mutant and found that the hypocotyl and petiole EOD-FR and shade avoidance are completely disrupted. This work shows that YUCCA auxin biosynthetic genes are essential for detectable shade avoidance and that YUCCA genes are important for petiole shade avoidance.

Author Comment

This version changes in response to reviewer comments at PeerJ. The manuscript has been updated to more clearly describe experimental conditions. We also better distinguish between end-of-day far-red (EOD-FR) and low red:fr (R:FR) treatments and fixed a number of minor errors.

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Patricia Müller-Moulé conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Kazunari Nozue performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Melissa L Pytlak conceived and designed the experiments, performed the experiments, reviewed drafts of the paper.

Christine M Palmer performed the experiments, reviewed drafts of the paper.

Michael F Covington contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Andreah D Wallace performed the experiments, reviewed drafts of the paper.

Stacey L Harmer contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Julin N Maloof conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

Raw data and code for making figures is at Github.com and accessible at https://github.com/MaloofLab/Mueller-Moule-PeerJ-2016

Funding

This work was funded by National Science Foundation (NSF) grant DBI-0227103 and IOS-0923752 to J.N.M. and Cynthia Weinig, UC Davis funds to J.N.M. ,United States Department of Agriculture NIFA project (http://www.csrees.usda.gov/; CA-D-PLB-7226-H) to JNM, USDA National Research Initiative grant number 2004-35100-14903 to M.F.C., and NIH grant GM069418 and NSF grant IOB 0315738 to S.L.H. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance
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