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Isolation and characterization of 7 microsatellite loci for the Florida Burrowing Owl (Athene cunicularia floridana)

, , , ,
1 Department of Biology, Hofstra University, Hempstead, New York, United States
2 UF Veterinary Forensic Program, W.R. Maples Center for Forensic Medicine, University of Florida, Gainesville, Florida, United States
3 Graduate Program in Environmental Science and Department of Biology, Pace University Pleasantville/Briarcliff, Pleasantville, New York, United States
DOI
10.7287/peerj.preprints.1927v1
Published
Accepted
Subject Areas
Conservation Biology, Ecology, Genetics
Keywords
Burrowing Owl, Raptor, Microsatellites, Genetic Variation
Copyright
© 2016 Laxer et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Laxer A, Krause M, Clark AM, Grigione MM, Sarno R. 2016. Isolation and characterization of 7 microsatellite loci for the Florida Burrowing Owl (Athene cunicularia floridana) PeerJ Preprints 4:e1927v1

Abstract

Despite long-term scientific interest in the Florida Burrowing Owl, little is known about key aspects of its ecology that likely influence population persistence. This is a cause of alarm considering that the Florida Burrowing Owl has been designated a “Species of Special Concern” since 1979. Therefore, seven microsatellite loci were developed and characterized for 48 Florida Burrowing Owls. All loci were polymorphic and six of them differed significantly from Hardy-Weinberg expectations. Although preliminary, the number of loci out of HW equilibrium may result from low population size and resultant non-random mating that could be caused by habitat fragmentation as development has ensued.

Author Comment

This is a preprint submission to PeerJ Preprints.

Supplemental Information

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Alison Laxer performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Maureen Krause conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Ann Marie Clark conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Melissa M Grigione reviewed drafts of the paper, trapped owls and collected blood samples.

Ronald Sarno conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, trapped owls and collected blood samples.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

University of South Florida IACUC #W:223

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

United States Department of the Interior, US Geological Survey.Permit Number 22418

Florida Fish and Wildlife Conservation Commission Permit Number WB03367

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a Supplemental Dataset

Funding

Partial funding for this work was provided by an Undergraduate Summer Fellowship to Alison Laxer and a Hofstra University Faculty Research Award to Ronald Sarno. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Isolation and characterization of 7 microsatellite loci for the Florida Burrowing Owl (Athene cunicularia floridana)
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