Association between osteoporosis and polymorphisms of the bone estrogen receptor 1, calcitonin receptor genes in Mongolian postmenopausal women
- Published
- Accepted
- Subject Areas
- Genetics, Gynecology and Obstetrics
- Keywords
- postmenopausal women, osteoporosis, estrogen receptor 1, calcitonin receptor
- Copyright
- © 2014 Lkhagvasuren et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Cite this article
- 2014. Association between osteoporosis and polymorphisms of the bone estrogen receptor 1, calcitonin receptor genes in Mongolian postmenopausal women. PeerJ PrePrints 2:e191v1 https://doi.org/10.7287/peerj.preprints.191v1
Abstract
In this study, we have investigated the association between osteoporosis and estrogen receptor 1 (ER1) 397 T>C, and calcitonin receptor (CALCR) 1340 T>C polymorphisms. Genomic DNA was obtained from 104 persons (52 osteoporotic and 52 healthy controls). Genomic DNA was extracted from EDTA-preserved peripheral venous blood of patients and controls and analyzed by PCR-RFLP. As a result, there was no statistically significant difference in the genotype and allele frequencies of patients and controls for ER1 397 T>C and CALCR 1340 T>C polymorphisms. ER1 CC and TC single nucleotides genotypes compared with TT genotypes was found more significantly women with osteoporosis [p=0.016; p=0.0046, OR=2.66; 0.44, 95% CI 1.185-5.988; 0.199-0.991)]. There was no statistically significant difference in the genotype and allele frequencies of patient and controls for ER1 combined nucleotides [p=0,148, OR=1.051, 95% CI (0.993–1.112)]. Our study showed that CALCR genes single and combined nucleotides genotypes were not significant women with osteoporotic and healthy postmenopausal women.
Author Comment
This study was first performed on the detection of osteoporosis factors correlating with genotypes of ER1 and CALCR among Mongolian postmenopausal women.
Supplemental Information
Table 1
Single nucleotide polymorphisms (SNPs) investigated in this study and PCR primers for the genotyping of ER1 and CALCR.
Table 3
Comparison of genotype and allele frequencies of ER1 and CALCR gene polymorphisms between osteoporotic patients and controls
Figure 1
Determination of ER1 and CALCR gene polymorphisms by PCR-RFLP. (a) ER1 genotypes, 25;19;55;36;03;28;53-sample ID number, SM-size marker 100 bp (b) CALCR genotypes, 98;239;252;211;255;124;157;115-sample ID number, SM-size marker 100 bp