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DNA cloning is a simple, straightforward process. It has been put into practice as a standard lab procedure for cloning the gene of interest for specific protein expression and other molecular or cellular studies. The present study is the first of its kind to hypothesize the formation of interfering recombinant plasmid during a DNA cloning reaction that is believed to interfere with the protein or gene expression. This might lead to a false conclusion in a cellular study and also may lower the protein expression during the production stage.