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Supplemental Information

Supplementary information, Figure 1

Neighbour-joining phylogeny for Bulimulus species, based on 654bp cytochrome oxidase I mitochondrila DNA. Bootstrap values of 70 and above are presented to the left of the nodes indicated by black dots. Scale bar in substitutions/site.

DOI: 10.7287/peerj.preprints.1716v1/supp-1

Supplementary information, Figure 2

Maximum-parsimony phylogeny for Bulimulus species, based on 654bp cytochrome oxidase I mitochondrila DNA. Bootstrap values of 90 and above are presented to the left of the nodes indicated by black dots.

DOI: 10.7287/peerj.preprints.1716v1/supp-2

Supplementary information, Table 1

Delimitation of MOTUs using K2P distances and standard error at 3% threshold. gA and gB, Bulimulus guadalupensis group A respectively B.

DOI: 10.7287/peerj.preprints.1716v1/supp-3

Supplementary information, Table 2

Delimitation of MOTUs using K2P distances and standard error at 4% threshold. gA and gB, Bulimulus guadalupensis group A respectively B.

DOI: 10.7287/peerj.preprints.1716v1/supp-4

Supplementary information, Table 3

Species delimiting as implemented in Geneious, using ML and BI for both the total dataset and a subgroup of MOTUs. Closest species, Intraspecific distance, Interspecies distance, ratio of Intra/Interspecific, P ID(strict), Rosenberg’s Pab, and Rodrigo’s P(RD) are indicated. Colours code for significance. c, d, g, gr, hu, s, and sp. correspond with the respective taxon names; NA, not applicable.

DOI: 10.7287/peerj.preprints.1716v1/supp-5

Supplementary information, Table 4

Mean p-distances between and within (diagonal) the different MOTUs based on the dataset analysed. EDS, Estimates of Evolutionary Divergence between Sequences; NEDGS, Net Evolutionary Divergence between Group of Sequences. c, d, g, gr, hu, s, and sp. correspond with the respective taxon names; n/c, not calculated. Colours code the corresponding groups.

DOI: 10.7287/peerj.preprints.1716v1/supp-6

Supplementary information, Table 5

Results for different combinations of MOTUs, using rooted trees of both ML and BI analyses, in terms of genealogical sorting index and corresponding p-values based on a permutation test of 10,000 replicates. Colours code the corresponding groups.

DOI: 10.7287/peerj.preprints.1716v1/supp-7

Additional Information

Competing Interests

The author declares that he has no competing interests.

Author Contributions

Abraham SH Breure conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank; accession numbers have been provided and sequences have been published

Data Deposition

The following information was supplied regarding data availability:

All raw data are either published (GenBank) or provided as supplementary files

Funding

The author received no funding for this work.


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