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Supplemental Information

Table 1

Measures of reliability for mean exercise and peak physiological variables during the HST (n = 6).

DOI: 10.7287/peerj.preprints.1513v1/supp-1

Table 2

Measures of reliability for mHSP70 (MFI A.U) for 5 repeated resting measurements (n = 5), prior to and after each HST (n = 6).

DOI: 10.7287/peerj.preprints.1513v1/supp-2

Table 3

Measures of reliability in normoxic (n = 6) and hypoxic (n = 6) conditions during the time trial. Data are calculated from the mean TT power output (Watts) and heart rate.

DOI: 10.7287/peerj.preprints.1513v1/supp-3

Figure 1: A typical flow cytometry file following staining with mHSP70 positive and negative antibodies

A typical flow cytometry profile showing a) forward side scatter (FSC-H/SSC-H) with gated monocytes and b) florescence intensity (FL1-H nm; FITC stained samples on a linear scale) of monocytes incubated with isotype matched negative controls (black line) and anti-HSP70 (green line) antibodies

DOI: 10.7287/peerj.preprints.1513v1/supp-4

Figure 2: Cardiorespiratory responses to the repeated hypoxic tests

Mean ± SD heart rate and SpO2 (a), VE (b), VO2 (c), and VCO2 (d) during the 3 HST’s (n = 6). No differences were observed across trials (p > 0.05).

DOI: 10.7287/peerj.preprints.1513v1/supp-5

Figure 3: mHSP70 responses to hypoxic exercise

Microsoft Word - Lee_Thake_reliability_hypoxia_2015.docx Repeat measurements of Monocyte HSP70 (n = 5) without (a) and with (b; n = 5) a HST in the previous 7 days. Panel c illustrates the reliable inverse response after exercise mHSP70 has with resting mHSP70. Lines show individual data and the bars represent the group mean.

DOI: 10.7287/peerj.preprints.1513v1/supp-6

Figure 4. Performance time and physiological data during repeated normoxic and hypoxic time trials

Mean ± SD performance time, average power output at each km, and average HR for each km in both the NORM (n = 6, panel a, b, c) and HYP (n = 6, panel d, e, f) throughout the 16.1 km time trial.

DOI: 10.7287/peerj.preprints.1513v1/supp-7

Additional Information

Competing Interests

The authors declare no conflicts of interest

Author Contributions

Ben J Lee conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Charles D Thake conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Coventry University Local Ethics Committee. REF P2566/6420.

Data Deposition

The following information was supplied regarding data availability:

The raw data is supplied as a supplemental file.

Funding

Aspects of this work were funded by a studentship awarded to Dr B J Lee by the Centre for Applied Biological and Exercise Sciences, Coventry University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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