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Supplemental Information

Map of sampling locations

Sample site markers are scaled to represent the mean number of protosteloid amoebae fruiting bodies encountered for each line of substrate observed from that site. N = species richness observed at each major latitudinal range.

DOI: 10.7287/peerj.preprints.136v2/supp-1

Collection site locations and characteristics

Supplementary table 1 - Collection site locations and characteristics. Number of lines plated refers to the number of lines of substrate observed from each site.

DOI: 10.7287/peerj.preprints.136v2/supp-2

Summary of species observations

Table 1 - Summary of species observations. A=abundant, C=common, O=occasional, R=rare *p<0.05; **p<0.01; ***p<0.001 (All tests: significant difference between Aerial and Ground litter abundance; one-way ANOVA test)

DOI: 10.7287/peerj.preprints.136v2/supp-3

Species encounters along environmental variables

Regressions of all observations of fruiting bodies' richness and abundance against latitude, elevation, and annual rainfall.

DOI: 10.7287/peerj.preprints.136v2/supp-4

Rarefaction curve showing sampling effort

Figure 3 - Collection-based rarefaction curve. Sampling effort appears sufficient to uncover the diversity of protosteloid amoebae.

DOI: 10.7287/peerj.preprints.136v2/supp-5

Additional Information

Competing Interests

The authors declare no competing interests.

Author Contributions

Geoffrey Zahn analyzed the data, contributed reagents/materials/analysis tools, wrote the paper.

Steven L. Stephenson conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools.

Frederick W. Spiegel conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools.

Grant Disclosures

The following grant information was disclosed by the authors:

National Science Foundation (DEB-0316284)

National Geographic Society (6051-97 and 4732-92)


This project was partially funded by the National Science Foundation (DEB-0316284) and The National Geographic Society (6051-97 and 4732-92). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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