Bronze Contributor

5,390 Points

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Contributions by subject area

Bronze Contributor

Wenfa Ng

PeerJ Author

Summary

I am a citizen scientist with a focus on biology and biotechnology. General areas of interest to me is at the interface of engineering and biology. Educated as an environmental engineer at the National University of Singapore, I also have a research-based masters in chemical engineering from my alma mater. Multiple stints as teaching assistant during my tenure as masters student introduced me to the importance of educating the next generation of students. Drawing on research skills I learnt, I attempt to fill pedagogy gaps in encouraging critical thinking in students and designed an inquiry-based learning module based on MALDI-TOF mass spectrometry.

Biochemistry Bioengineering Biotechnology Environmental Sciences Microbiology

Past or current institution affiliations

National University of Singapore

Websites

PeerJ Contributions

Preprints 81

September 3, 2019 - Version: 2

MATLAB software for automated calculation of concentration of different compounds using extracted peak area from HPLC data file in pdf format

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27878v2

July 16, 2019 - Version: 2

MATLAB software for extracting protein name and sequence information from FASTA formatted proteome file

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27856v2

July 2, 2019 - Version: 1

Molecular weight calculator for organic compounds in biotechnology

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27836v1

June 5, 2019 - Version: 1

Cultivating the uncultivable environmental microbe using insights from whole genome sequence that define a growth medium

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27784v1

June 2, 2019 - Version: 1

Understanding the evolutionary origins of bacterial natural products with immunosuppressant properties

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27774v1

May 30, 2019 - Version: 1

Proposal of conceptual model for the mechanism underpinning evolution of operon as pathway gene organizer in bacterial genome

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27767v1

May 29, 2019 - Version: 1

Setting a price for carbon for implementing a carbon tax or a cap and trade system for controlling carbon dioxide emissions

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27765v1

May 28, 2019 - Version: 1

Fuel efficiency standards’ roles in reducing greenhouse gas emissions and air pollutants

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27763v1

May 27, 2019 - Version: 1

Energy use in water purification as criterion for selecting drinking water treatment technologies

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27761v1

May 26, 2019 - Version: 1

Understanding the chemical basis for the preferential ionization of specific biomolecules in mass spectrometry analysis of microbial cells

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27759v1

May 23, 2019 - Version: 1

Understanding gene transfer between endosymbiotic organisms

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27752v1

May 20, 2019 - Version: 1

Possibility of tuning the differentiation state of tumor associated macrophages towards tumor controlling phenotypes

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27747v1

May 16, 2019 - Version: 1

Possibility of understanding metabolic syndrome by probing dysregulation of metabolic and signaling network in bacteria

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27738v1

May 12, 2019 - Version: 1

Exploring lowering the optimal growth temperature of Escherichia coli in biotechnology applications

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27723v1

May 10, 2019 - Version: 1

In silico experiments validate that twenty nucleotide spacer sequence provides precise targeting of bacterial genes in CRISPR-Cas9

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27719v1

May 8, 2019 - Version: 1

Biochemical traits that Escherichia coli evolved during its adaption to the gastrointestinal tract of humans

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27713v1

May 6, 2019 - Version: 1

Possible epigenetic influence on cellular differentiation and lineage segregation in Escherichia coli

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27704v1

May 2, 2019 - Version: 1

Possible differences in efficiency of guide RNA with different spacer sequence in CRISPR knock-down or knock-out of particular gene

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27701v1

April 29, 2019 - Version: 1

Merits of constant expression of CRISPR loci in adaptive immunity of bacteria

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27691v1

April 25, 2019 - Version: 1

Accessing the metabolism of viable but non-culturable (VBNC) microbes

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27683v1

April 22, 2019 - Version: 1

Snippets of virus integrated into human genome suggests possibility of CRISPR-like adaptive immune system in human cells

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27675v1

April 20, 2019 - Version: 1

Search for receptors in immune cells that bind cancer cell antigens and their activation in silent cases

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27670v1

April 17, 2019 - Version: 1

Possibility of detecting cancer using liquid chromatography mass spectrometry profiling of cancer signature metabolome in blood

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27660v1

April 15, 2019 - Version: 1

Bacterial Type VI secretion system could have evolved from co-opted tail sheath tube of bacteriophages

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27652v1

April 11, 2019 - Version: 1

Possibility of correlating mutational profile of cancer with drugs suitable for treatment

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27648v1

April 8, 2019 - Version: 1

Elaborate mechanism for the synthesis of lipopolysaccharide points to its functional importance in Gram-negative bacteria

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27642v1

April 4, 2019 - Version: 1

Blood biopsy profiles circulating tumour cells which hold clues to cancer treatment

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27635v1

April 2, 2019 - Version: 1

Difficulty in dislodging biofilms from inner surface of drinking water pipelines

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27628v1

January 14, 2019 - Version: 1

Scientific possibilities in associating microbiome to specific diseases and its bioinformatic and experimental challenges

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27483v1

January 3, 2019 - Version: 1

Highlight on fusing multiple chromosomes in yeast into a single chromosome

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27462v1

August 15, 2018 - Version: 1

Light-based patterning of bacterial cells through induction of biofilm formation processes

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27117v1

July 6, 2018 - Version: 1

Type II diabetes may affect stem cell niche resulting in down regulation of glucose transporters and insulin receptors in cells

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27024v1

June 29, 2018 - Version: 1

Effect of different aeration and polyethylene glycol concentration on growth of Escherichia coli DH5α in a 1L bioreactor

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.27014v1

June 10, 2018 - Version: 1

High mass resolution MALDI-TOF MS for profiling biomolecules in mixtures

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26983v1

June 2, 2018 - Version: 1

Avoiding CRISPR: Plasmid design for genetic engineering

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26975v1

June 1, 2018 - Version: 1

Growth of Pseudomonas protegens Pf-5 in M9 minimal salts medium

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26970v1

May 22, 2018 - Version: 1

Effect of glucose and ammonium chloride supplementation and phosphate buffer on Escherichia coli DH5α growth in LB Lennox medium

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26949v1

May 12, 2018 - Version: 2

Conserved mass peaks in MALDI-TOF mass spectra of bacterial species at the genus and species levels

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3524v2

May 5, 2018 - Version: 1

Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26914v1

April 28, 2018 - Version: 1

Effect of temperature on growth of Pseudomonas protegens Pf-5 and Pseudomonas aeruginosa PRD-10 in LB Lennox medium

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26901v1

April 23, 2018 - Version: 1

Theoretical ribosomal protein mass fingerprint database

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26878v1

April 13, 2018 - Version: 2

Drastic decline in optical density during stationary phase meant that Bacillus subtilis NRS-762 is not suitable as model organism in microbial survivability studies

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3103v2

March 31, 2018 - Version: 1

Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26817v1

March 23, 2018 - Version: 6

Microbes in deionized and tap water: Implications for maintenance of laboratory water production system

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.181v6

March 14, 2018 - Version: 1

Zeta potential of Escherichia coli DH5α grown in different growth media

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26697v1

March 6, 2018 - Version: 1

Surface charge characteristics of Bacillus subtilis NRS-762 cells

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26626v1

March 1, 2018 - Version: 1

High cell density cultivation of Escherichia coli DH5α in shake flasks with a new formulated medium

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26598v1

February 21, 2018 - Version: 6

Colourless agar for enhancing colour contrast between microbial colonies and agar

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.89v6

February 13, 2018 - Version: 1

Existence of theoretical ribosomal protein mass fingerprints in bacteria, archaea and eukaryotes

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.26511v1

February 10, 2018 - Version: 4

Bacterial surface charge in “layers”: revealed by wash buffers of different ionic strength

Wenfa Ng, Yen-Peng Ting

https://doi.org/10.7287/peerj.preprints.2086v4

January 1, 2018 - Version: 1

Theoretical ribosomal protein mass distribution of Pseudomonas aeruginosa PAO1

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3500v1

December 11, 2017 - Version: 1

Average deviation for measuring variation in data in small samples (n < 5)

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3460v1

November 21, 2017 - Version: 1

Inability to completely remove trace contaminants from drinking water by adsorption

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3423v1

October 16, 2017 - Version: 3

Graphical illustration for explaining mass spectrum fingerprinting in microbial identification

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.580v3

September 24, 2017 - Version: 2

Ammonium interference reduced copper uptake by formaldehyde-crosslinked Sargassum sp. seaweed

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.1228v2

September 18, 2017 - Version: 1

Relevance of single cell and single molecule studies at different biological and physical length scales

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3261v1

September 12, 2017 - Version: 1

Transparent PET optical window for microfluidic cell culture device

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3245v1

September 8, 2017 - Version: 1

Segmented co-infection of tree bark by different fungus

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3239v1

August 26, 2017 - Version: 1

Single cell transcriptomics view of cell lineage, cell fate and cellular differentiation

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3193v1

August 22, 2017 - Version: 7

Zeta potential of bacterial cells: Effect of wash buffers

Wenfa Ng, Yen-Peng Ting

https://doi.org/10.7287/peerj.preprints.110v7

August 22, 2017 - Version: 6

Formulation of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks

Wenfa Ng, Yen-Peng Ting

https://doi.org/10.7287/peerj.preprints.1406v6

August 16, 2017 - Version: 1

Preprints as medium for communicating new ideas, hypotheses, data, analysis and beyond

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.3154v1

June 9, 2017 - Version: 5

Direct dilution of cell aliquot for high temporal resolution bacterial cell surface charge measurement

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.91v5

June 7, 2017 - Version: 5

Development of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks: Part 2

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.117v5

June 5, 2017 - Version: 6

Development of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks: Part 1

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.115v6

May 6, 2017 - Version: 1

Understanding evolutionary dynamics of phosphorylation with fungal species as model system

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2966v1

March 29, 2017 - Version: 3

Socratic approach for unlocking creative potential in undergraduate research students

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.359v3

March 21, 2017 - Version: 6

Patterning bacteria on agar for understanding distance mediated cell to cell signaling and metabolic exchanges

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.349v6

February 13, 2017 - Version: 4

Brief history of computational chemistry: Three distinct eras and the relative importance of theoretical insights and computing power in advancing the field

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.365v4

February 10, 2017 - Version: 1

Transmissible cancer in marine bivalves: Implications for mode of cancer transmission

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2800v1

February 6, 2017 - Version: 6

Mouse xenograft models for elucidating drug resistance mechanisms

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.1049v6

January 26, 2017 - Version: 3

Cost hurdles to open access publishing: A citizen scientist perspective

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2463v3

January 13, 2017 - Version: 1

Splice variants’ role in mediating different disease states in tissue requiring ion transduction through calcium channel for function

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2717v1

January 4, 2017 - Version: 1

Poleward and vertical migration of animals under climate change highlights possible environmental effects on social behaviour

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2696v1

January 3, 2017 - Version: 1

Possible variation in microbiota community structure and composition in same species bred in zoos and a wildlife refuge, and its implications on reproductive success

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2686v1

December 12, 2016 - Version: 1

Defining and redefining its role in biology: Synthetic biology as an emerging field at the interface of engineering and biology

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2634v1

September 12, 2016 - Version: 3

Identifying microbes from environment water samples in a discovery-based learning module

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.399v3

August 19, 2016 - Version: 1

White discoloration on leaves and flower-like outgrowth on stem: A new species or hormonal disequilibrium?

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.2375v1

August 17, 2016 - Version: 5

What drives computation chemistry forward: theory or computing power?

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.552v5

July 28, 2016 - Version: 5

Towards extracellular secretion of poly-β-hydroxybutyric acid (PHB) biodegradable polymer

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.534v5

September 23, 2015 - Version: 3

Possible odour-mediated attraction of flies to Bacillus subtilis NRS-762 stationary phase culture

Wenfa Ng

https://doi.org/10.7287/peerj.preprints.541v3