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  1. (July 8, 2015) A peer-reviewed version of this preprint has been published in PLOS ONE.

Supplemental Information

S1 Table. Information on Dinocras cephalotes specimen weights (in milligram) for experiment I

DOI: 10.7287/peerj.preprints.1023v1/supp-1

S2 Table. Information on specimen weights (in milligram) for experiment II

DOI: 10.7287/peerj.preprints.1023v1/supp-2

S3 Table. MOTU assignment to individual specimens in experiment II

DOI: 10.7287/peerj.preprints.1023v1/supp-3

S1 Figure. Fusion COI Primers developed in this study

Fusion primer can be directly loaded onto the MiSeq system and universal primers modified or replaced.

DOI: 10.7287/peerj.preprints.1023v1/supp-4

S2 Figure. Increase of diversity by parallel sequencing. By sequencing forward and reverse primers together, sequence diversity and thus read quality is increased

By sequencing forward and reverse primers together, sequence diversity and thus read quality is increased.

DOI: 10.7287/peerj.preprints.1023v1/supp-5

S3 Figure. Number of reads excluded in data processing steps

Includes flow charts of the bioinformatics processing of experiment I (A) and experiment II (B).

DOI: 10.7287/peerj.preprints.1023v1/supp-6

S4 Figure. Reads in each replicate after demultiplexing

Data from experiment I (A) and experiment II (B).

DOI: 10.7287/peerj.preprints.1023v1/supp-7

S5 Figure. Experiment I: sequences per specimen

Normalised sequence abundance for each stonefly.

DOI: 10.7287/peerj.preprints.1023v1/supp-8

S6 Figure. Experiment I: sequencing artefacts

Sequence matches are shown for three individual specimens, including h28 and h13 that are affected by sequencing artefacts.

DOI: 10.7287/peerj.preprints.1023v1/supp-9

S7 Figure. Experiment I: Variability in sequence abundance

Variability in sequence abundance between the ten replicates as well as dependence on specimen biomass.

DOI: 10.7287/peerj.preprints.1023v1/supp-10

S8 Figure. Experiment I: Sequence abundance depended on specimen biomass

Mean normalised sequence abundance of all ten replicates, including standard errors.

DOI: 10.7287/peerj.preprints.1023v1/supp-11

S9 Figure. Experiment II: OTUs assigned to taxa

Detailed overview of all 213 OTUs and their taxonomic identification using the BOLD database.

DOI: 10.7287/peerj.preprints.1023v1/supp-12

Additional Information

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Vasco Elbrecht conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Florian Leese conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The Illumina sequencing data are available via the Short Read Archive (accession numbers SRS731403 and SRS733820 ).

Funding

This work was supported by a grant of the Kurt Eberhard Bode Foundation to FL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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