S1 Table. Information on Dinocras cephalotes specimen weights (in milligram) for experiment I
S2 Table. Information on specimen weights (in milligram) for experiment II
S3 Table. MOTU assignment to individual specimens in experiment II
S1 Figure. Fusion COI Primers developed in this study
Fusion primer can be directly loaded onto the MiSeq system and universal primers modified or replaced.
S2 Figure. Increase of diversity by parallel sequencing. By sequencing forward and reverse primers together, sequence diversity and thus read quality is increased
By sequencing forward and reverse primers together, sequence diversity and thus read quality is increased.
S3 Figure. Number of reads excluded in data processing steps
Includes flow charts of the bioinformatics processing of experiment I (A) and experiment II (B).
S4 Figure. Reads in each replicate after demultiplexing
Data from experiment I (A) and experiment II (B).
S5 Figure. Experiment I: sequences per specimen
Normalised sequence abundance for each stonefly.
S6 Figure. Experiment I: sequencing artefacts
Sequence matches are shown for three individual specimens, including h28 and h13 that are affected by sequencing artefacts.
S7 Figure. Experiment I: Variability in sequence abundance
Variability in sequence abundance between the ten replicates as well as dependence on specimen biomass.
S8 Figure. Experiment I: Sequence abundance depended on specimen biomass
Mean normalised sequence abundance of all ten replicates, including standard errors.