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Supplemental Information

Figure S1. Estimation of the half-life

The half-life of the viability for BL21-AI(IY,1amb-immE3) was estimated in the absence of IY. A single fitted curve was generated using an exponential decrease model. The half-life was calculated from the equation for the fitted curve.

DOI: 10.7287/peerj.preprints.1001v1/supp-1

Figure S2. Estimation of the growth rates

The growth rates were estimated for BL21-AI(IY,1amb-immE3), the parent strain carrying pSH350, and for a vector control carrying pBR322 instead of pSH350. A single fitted curve was generated for each strain using an exponential increase model. The growth rates were calculated from the equations for the fitted curves.

DOI: 10.7287/peerj.preprints.1001v1/supp-2

Table S1. List of PCR primers

DOI: 10.7287/peerj.preprints.1001v1/supp-3

Data S1. Data set for Figure 2B

DOI: 10.7287/peerj.preprints.1001v1/supp-4

Data S2. Data set for Figure 2C

DOI: 10.7287/peerj.preprints.1001v1/supp-5

Data S3. Data set for fluctuation assay

DOI: 10.7287/peerj.preprints.1001v1/supp-6

Additional Information

Competing Interests

The author declares they have no competing interests.

Author Contributions

Yusuke Kato conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.


This work was supported by JSPS KAKENHI Grant number 25660281. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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