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Wenfa Ng
Bronze Contributor
5,390 Points

Contributions by role

Preprint Author 5,390

Contributions by subject area

Bioengineering
Bioinformatics
Cell Biology
Genomics
Histology
Biotechnology
Environmental Sciences
Biochemistry
Ecology
Microbiology
Molecular Biology
Science and Medical Education
Biophysics
Computational Biology
Computational Science
Biodiversity
Genetics
Science Policy
Animal Behavior
Conservation Biology
Zoology
Anatomy and Physiology
Marine Biology
Soil Science
Biogeography
Environmental Contamination and Remediation
Taxonomy
Ecosystem Science
Aquatic and Marine Chemistry
Ecohydrology
Environmental Impacts
Food, Water and Energy Nexus
Coupled Natural and Human Systems
Atmospheric Chemistry
Internal Medicine
Synthetic Biology
Freshwater Biology
Diabetes and Endocrinology
Metabolic Sciences
Immunology
Biogeochemistry
Statistics
Bronze Contributor

Wenfa Ng

PeerJ Author

Summary

I am a citizen scientist with a focus on biology and biotechnology. General areas of interest to me is at the interface of engineering and biology. Educated as an environmental engineer at the National University of Singapore, I also have a research-based masters in chemical engineering from my alma mater. Multiple stints as teaching assistant during my tenure as masters student introduced me to the importance of educating the next generation of students. Drawing on research skills I learnt, I attempt to fill pedagogy gaps in encouraging critical thinking in students and designed an inquiry-based learning module based on MALDI-TOF mass spectrometry.

Biochemistry Bioengineering Biotechnology Environmental Sciences Microbiology

Editing Journals

Past or current institution affiliations

National University of Singapore

Websites

  • Wenfa Ng at figshare
  • Masters' thesis
  • Looking at life with a scientific lens
  • Google Scholar
  • Social Science Research Network, SSRN

PeerJ Contributions

  • Preprints 81
September 3, 2019 - Version: 2
MATLAB software for automated calculation of concentration of different compounds using extracted peak area from HPLC data file in pdf format
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27878v2
July 16, 2019 - Version: 2
MATLAB software for extracting protein name and sequence information from FASTA formatted proteome file
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27856v2
July 2, 2019 - Version: 1
Molecular weight calculator for organic compounds in biotechnology
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27836v1
June 5, 2019 - Version: 1
Cultivating the uncultivable environmental microbe using insights from whole genome sequence that define a growth medium
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27784v1
June 2, 2019 - Version: 1
Understanding the evolutionary origins of bacterial natural products with immunosuppressant properties
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27774v1
May 30, 2019 - Version: 1
Proposal of conceptual model for the mechanism underpinning evolution of operon as pathway gene organizer in bacterial genome
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27767v1
May 29, 2019 - Version: 1
Setting a price for carbon for implementing a carbon tax or a cap and trade system for controlling carbon dioxide emissions
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27765v1
May 28, 2019 - Version: 1
Fuel efficiency standards’ roles in reducing greenhouse gas emissions and air pollutants
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27763v1
May 27, 2019 - Version: 1
Energy use in water purification as criterion for selecting drinking water treatment technologies
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27761v1
May 26, 2019 - Version: 1
Understanding the chemical basis for the preferential ionization of specific biomolecules in mass spectrometry analysis of microbial cells
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27759v1
May 23, 2019 - Version: 1
Understanding gene transfer between endosymbiotic organisms
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27752v1
May 20, 2019 - Version: 1
Possibility of tuning the differentiation state of tumor associated macrophages towards tumor controlling phenotypes
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27747v1
May 16, 2019 - Version: 1
Possibility of understanding metabolic syndrome by probing dysregulation of metabolic and signaling network in bacteria
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27738v1
May 12, 2019 - Version: 1
Exploring lowering the optimal growth temperature of Escherichia coli in biotechnology applications
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27723v1
May 10, 2019 - Version: 1
In silico experiments validate that twenty nucleotide spacer sequence provides precise targeting of bacterial genes in CRISPR-Cas9
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27719v1
May 8, 2019 - Version: 1
Biochemical traits that Escherichia coli evolved during its adaption to the gastrointestinal tract of humans
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27713v1
May 6, 2019 - Version: 1
Possible epigenetic influence on cellular differentiation and lineage segregation in Escherichia coli
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27704v1
May 2, 2019 - Version: 1
Possible differences in efficiency of guide RNA with different spacer sequence in CRISPR knock-down or knock-out of particular gene
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27701v1
April 29, 2019 - Version: 1
Merits of constant expression of CRISPR loci in adaptive immunity of bacteria
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27691v1
April 25, 2019 - Version: 1
Accessing the metabolism of viable but non-culturable (VBNC) microbes
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27683v1
April 22, 2019 - Version: 1
Snippets of virus integrated into human genome suggests possibility of CRISPR-like adaptive immune system in human cells
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27675v1
April 20, 2019 - Version: 1
Search for receptors in immune cells that bind cancer cell antigens and their activation in silent cases
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27670v1
April 17, 2019 - Version: 1
Possibility of detecting cancer using liquid chromatography mass spectrometry profiling of cancer signature metabolome in blood
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27660v1
April 15, 2019 - Version: 1
Bacterial Type VI secretion system could have evolved from co-opted tail sheath tube of bacteriophages
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27652v1
April 11, 2019 - Version: 1
Possibility of correlating mutational profile of cancer with drugs suitable for treatment
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27648v1
April 8, 2019 - Version: 1
Elaborate mechanism for the synthesis of lipopolysaccharide points to its functional importance in Gram-negative bacteria
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27642v1
April 4, 2019 - Version: 1
Blood biopsy profiles circulating tumour cells which hold clues to cancer treatment
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27635v1
April 2, 2019 - Version: 1
Difficulty in dislodging biofilms from inner surface of drinking water pipelines
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27628v1
January 14, 2019 - Version: 1
Scientific possibilities in associating microbiome to specific diseases and its bioinformatic and experimental challenges
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27483v1
January 3, 2019 - Version: 1
Highlight on fusing multiple chromosomes in yeast into a single chromosome
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27462v1
August 15, 2018 - Version: 1
Light-based patterning of bacterial cells through induction of biofilm formation processes
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27117v1
July 6, 2018 - Version: 1
Type II diabetes may affect stem cell niche resulting in down regulation of glucose transporters and insulin receptors in cells
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27024v1
June 29, 2018 - Version: 1
Effect of different aeration and polyethylene glycol concentration on growth of Escherichia coli DH5α in a 1L bioreactor
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.27014v1
June 10, 2018 - Version: 1
High mass resolution MALDI-TOF MS for profiling biomolecules in mixtures
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26983v1
June 2, 2018 - Version: 1
Avoiding CRISPR: Plasmid design for genetic engineering
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26975v1
June 1, 2018 - Version: 1
Growth of Pseudomonas protegens Pf-5 in M9 minimal salts medium
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26970v1
May 22, 2018 - Version: 1
Effect of glucose and ammonium chloride supplementation and phosphate buffer on Escherichia coli DH5α growth in LB Lennox medium
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26949v1
May 12, 2018 - Version: 2
Conserved mass peaks in MALDI-TOF mass spectra of bacterial species at the genus and species levels
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3524v2
May 5, 2018 - Version: 1
Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26914v1
April 28, 2018 - Version: 1
Effect of temperature on growth of Pseudomonas protegens Pf-5 and Pseudomonas aeruginosa PRD-10 in LB Lennox medium
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26901v1
April 23, 2018 - Version: 1
Theoretical ribosomal protein mass fingerprint database
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26878v1
April 13, 2018 - Version: 2
Drastic decline in optical density during stationary phase meant that Bacillus subtilis NRS-762 is not suitable as model organism in microbial survivability studies
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3103v2
March 31, 2018 - Version: 1
Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26817v1
March 23, 2018 - Version: 6
Microbes in deionized and tap water: Implications for maintenance of laboratory water production system
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.181v6
March 14, 2018 - Version: 1
Zeta potential of Escherichia coli DH5α grown in different growth media
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26697v1
March 6, 2018 - Version: 1
Surface charge characteristics of Bacillus subtilis NRS-762 cells
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26626v1
March 1, 2018 - Version: 1
High cell density cultivation of Escherichia coli DH5α in shake flasks with a new formulated medium
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26598v1
February 21, 2018 - Version: 6
Colourless agar for enhancing colour contrast between microbial colonies and agar
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.89v6
February 13, 2018 - Version: 1
Existence of theoretical ribosomal protein mass fingerprints in bacteria, archaea and eukaryotes
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.26511v1
February 10, 2018 - Version: 4
Bacterial surface charge in “layers”: revealed by wash buffers of different ionic strength
Wenfa Ng, Yen-Peng Ting
https://doi.org/10.7287/peerj.preprints.2086v4
January 1, 2018 - Version: 1
Theoretical ribosomal protein mass distribution of Pseudomonas aeruginosa PAO1
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3500v1
December 11, 2017 - Version: 1
Average deviation for measuring variation in data in small samples (n < 5)
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3460v1
November 21, 2017 - Version: 1
Inability to completely remove trace contaminants from drinking water by adsorption
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3423v1
October 16, 2017 - Version: 3
Graphical illustration for explaining mass spectrum fingerprinting in microbial identification
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.580v3
September 24, 2017 - Version: 2
Ammonium interference reduced copper uptake by formaldehyde-crosslinked Sargassum sp. seaweed
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.1228v2
September 18, 2017 - Version: 1
Relevance of single cell and single molecule studies at different biological and physical length scales
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3261v1
September 12, 2017 - Version: 1
Transparent PET optical window for microfluidic cell culture device
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3245v1
September 8, 2017 - Version: 1
Segmented co-infection of tree bark by different fungus
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3239v1
August 26, 2017 - Version: 1
Single cell transcriptomics view of cell lineage, cell fate and cellular differentiation
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3193v1
August 22, 2017 - Version: 7
Zeta potential of bacterial cells: Effect of wash buffers
Wenfa Ng, Yen-Peng Ting
https://doi.org/10.7287/peerj.preprints.110v7
August 22, 2017 - Version: 6
Formulation of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks
Wenfa Ng, Yen-Peng Ting
https://doi.org/10.7287/peerj.preprints.1406v6
August 16, 2017 - Version: 1
Preprints as medium for communicating new ideas, hypotheses, data, analysis and beyond
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.3154v1
June 9, 2017 - Version: 5
Direct dilution of cell aliquot for high temporal resolution bacterial cell surface charge measurement
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.91v5
June 7, 2017 - Version: 5
Development of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks: Part 2
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.117v5
June 5, 2017 - Version: 6
Development of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks: Part 1
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.115v6
May 6, 2017 - Version: 1
Understanding evolutionary dynamics of phosphorylation with fungal species as model system
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2966v1
March 29, 2017 - Version: 3
Socratic approach for unlocking creative potential in undergraduate research students
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.359v3
March 21, 2017 - Version: 6
Patterning bacteria on agar for understanding distance mediated cell to cell signaling and metabolic exchanges
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.349v6
February 13, 2017 - Version: 4
Brief history of computational chemistry: Three distinct eras and the relative importance of theoretical insights and computing power in advancing the field
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.365v4
February 10, 2017 - Version: 1
Transmissible cancer in marine bivalves: Implications for mode of cancer transmission
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2800v1
February 6, 2017 - Version: 6
Mouse xenograft models for elucidating drug resistance mechanisms
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.1049v6
January 26, 2017 - Version: 3
Cost hurdles to open access publishing: A citizen scientist perspective
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2463v3
January 13, 2017 - Version: 1
Splice variants’ role in mediating different disease states in tissue requiring ion transduction through calcium channel for function
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2717v1
January 4, 2017 - Version: 1
Poleward and vertical migration of animals under climate change highlights possible environmental effects on social behaviour
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2696v1
January 3, 2017 - Version: 1
Possible variation in microbiota community structure and composition in same species bred in zoos and a wildlife refuge, and its implications on reproductive success
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2686v1
December 12, 2016 - Version: 1
Defining and redefining its role in biology: Synthetic biology as an emerging field at the interface of engineering and biology
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2634v1
September 12, 2016 - Version: 3
Identifying microbes from environment water samples in a discovery-based learning module
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.399v3
August 19, 2016 - Version: 1
White discoloration on leaves and flower-like outgrowth on stem: A new species or hormonal disequilibrium?
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.2375v1
August 17, 2016 - Version: 5
What drives computation chemistry forward: theory or computing power?
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.552v5
July 28, 2016 - Version: 5
Towards extracellular secretion of poly-β-hydroxybutyric acid (PHB) biodegradable polymer
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.534v5
September 23, 2015 - Version: 3
Possible odour-mediated attraction of flies to Bacillus subtilis NRS-762 stationary phase culture
Wenfa Ng
https://doi.org/10.7287/peerj.preprints.541v3