Fecal microbiota in client-owned obese dogs changes after weight loss with a high-fiber-high-protein diet

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RT @sancbs: So glad! My article has been published today in @thePeerJ https://t.co/vmcWL3QDcB #Microbiology #VeterinaryMedicine #obesity #m…
Bermudez Sanchez et al. examine the fecal microbiota in client-owned obese dogs changes after weight loss with a high-fiber-high-protein diet. Read the full @thePeerJ article https://t.co/vpriws5oqF @UniTeramo @TAMU @LivUni https://t.co/pX2YsgRR5m
Microbiology

Main article text

 

Introduction

Materials & Methods

Study Animals, eligibility criteria and ethical considerations

Weight loss regimen

Fecal collection and DNA extraction

Quantitative PCR (qPCR) and Dysbiosis Index (DI)

16S rRNA gene sequencing

Analysis of sequences

Statistical analysis

Results

Animal population characteristics

qPCR and dysbiosis index

Changes in the fecal microbiota with weight loss in dogs with obesity

A short-term change in diet does not alter fecal microbiota

Discussion

Conclusions

Supplemental Information

Dogs enrolled in the study

DOI: 10.7717/peerj.9706/supp-1

Median percentage of relative abundance of the different bacterial populations detected in fecal samples of dogs with obesity before and after weight loss

DOI: 10.7717/peerj.9706/supp-2

Additional Information and Declarations

Competing Interests

The diets used in this study were manufactured by Royal Canin. Alexander J. German and Georgiana R.T. Woods are employees of the University of Liverpool but his academic post is funded by Royal Canin, part of Mars Petcare. Both have received financial remuneration and gifts for providing educational material, speaking at conferences, and consultancy work. Alexander J. Germans’s position at the University of Liverpool is funded by Royal Canin; Alexander J. German has also received financial remuneration and gifts for providing educational material, speaking at conferences, and consultancy work.

Rachel Pilla, Joerg M. Steiner, Jonathan A. Lidbury and Jan S. Suchodolski are employed by the Gastrointestinal Laboratory at Texas A&M University, which provides assay for intestinal function and microbiota analysis on a fee-for-service basis. Jan S. Suchodolski have also received consulting fees from Royal Canin.

Author Contributions

Sandra Bermudez Sanchez performed the experiments, analyzed the data, prepared figures and/or tables, wrote the paper and performed the statistical analysis, and approved the final draft.

Rachel Pilla, Joerg M. Steiner and Jonathan A. Lidbury analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Benjamin Sarawichitr performed the experiments, analyzed the data, prepared figures and/or tables, performed the statistical analysis, and approved the final draft.

Alessandro Gramenzi and Fulvio Marsilio conceived and designed the experiments, authored or reviewed drafts of the paper, and approved the final draft.

Georgiana R.T. Woods analyzed the data, authored or reviewed drafts of the paper, clinical assessment and management of patients, sample acquisition and storage, collection and collation of clinical data, and approved the final draft.

Alexander J. German conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, clinical assessment and management of patients, sample acquisition and storage, collection and collation of clinical data, and approved the final draft.

Jan S. Suchodolski conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the paper, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The study protocol was reviewed and approved by the University of Liverpool Veterinary Research Ethics Committee (Approval reference: RETH000353 and VREC793), the Royal Canin ethical review committee, and the WALTHAM ethical review committee. Owners of obese dogs gave informed consent in writing.

Data Availability

The following information was supplied regarding data availability:

Raw sequences are available in NCBI GenBank: PRJNA580258.

Funding

This work has received financial support from Mars Petcare (VCR10030) and from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement (No. 713714) to ESR 09 (Sandra Bermudez Sanchez). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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