Proteomic analysis of exudate of Cercospora armoraciae from Armoracia rusticana

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RT @thePeerJ: New study: Proteomic analysis of exudate of Cercospora armoraciae from Armoracia rusticana #proteome #Agriculturalscience Rea…
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Microbiology

Main article text

 

Introduction

Materials and Methods

Fungal materials

Pathogenicity assays

Library construction and sequencing

Raw read filtering

De novo assembly

Protein extraction

Protein digestion

nano-HPLC-MS/MS analysis

Database search

Protein functional annotation and enrichment analysis

Results

Pathogenicity tests

Illumina sequencing and de novo assembly of the C. armoraciae transcriptome

Protein identification

GO classification

KEGG pathway analysis

COG/KOG functional annotation

Discussion

Conclusions

Supplemental Information

De novo assembly sequences of Cercospora armoraciae.

DOI: 10.7717/peerj.9592/supp-1

List of proteins identified with LC-MS/MS in the exudates of Cercospora armoraciae.

DOI: 10.7717/peerj.9592/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Haining Wang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, and approved the final draft.

Songhong Wei conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Xiaohe Yang performed the experiments, prepared figures and/or tables, and approved the final draft.

Wei Liu conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Lijun Zhu conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data of RNA-seq are available at the National Center for Biotechnology Information Sequence Read Archive (SRA): SRR9163995.

The mass spec data are available in ProteomeXchange: PXD019663.

Funding

This work was supported by the earmarked fund for China Agriculture Research System (CARS-01) and the National Key R&D Program of China (2018YFD0300307). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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