Are improper kinetic models hampering drug development?

View article
PeerJ

Main article text

 

The Problem with Classical Inhibition Models

Empirical Modeling of Complex Inhibition Kinetics

Concluding Remarks

Supplemental Information

Gamma-secretase DAPT data fitting

DOI: 10.7717/peerj.649/supp-1

Description of constants used in the fitting

DOI: 10.7717/peerj.649/supp-2

Boxplot of the residuals associated with each data fitting

(A) Residuals produced by Eq. (10) with the published values, (B) residuals associated with the Eq. (10) after it was refit to the data and (C) residuals associated with Eq. (5). Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; outliers are represented by dots. Since Eq. (10) was not fit to background substrate concentrations, for A and B, n = 208, while for C (Eq. (5)) n = 230 sample points. This plot was generated using the web-tool BoxplotR (Spitzer et al., 2014).

DOI: 10.7717/peerj.649/supp-3

Additional Information and Declarations

Competing Interests

The author declares there are no competing interests.

Author Contributions

Ryan Walsh conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Funding

The author declares there was no funding for this work.

13 Citations 4,199 Views 1,092 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more