Isolation and identification of pathogenic fungi and oomycetes associated with beans and cowpea root diseases in Oman

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Microbiology

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Introduction

Materials and Methods

Survey and collection of samples

Isolation of fungi

Identification of fungi

Polymerase chain reaction and sequencing

Pathogenicity test

Data analysis

Results

Survey

Identification of fungi

Pathogenicity test on Phaseolus vulgaris

Pathogenicity test on Vigna unguiculata

Pathogenicity test on Vigna radiata

Pathogenicity test on Vicia faba

Discussion

Conclusions

Supplemental Information

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Asma Al-Jaradi conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Issa Al-Mahmooli performed the experiments, authored or reviewed drafts of the paper, approved the final draft.

Rhonda Janke conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Sajeewa Maharachchikumbura performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Nadiya Al-Saady conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Abdullah M. Al-Sadi conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

DNA sequencing data are provided in Table 1.

Data Availability

The following information was supplied regarding data availability:

Sequences are available in GenBank under the accession numbers described in Table 1 and the raw data is available as Data S1.

Funding

This study was funded by Sultan Qaboos University (IG/AGR/CROP/16/03) and the Oman Animal and Plant Genetic Resources Center (EG/AGR/CROP/16/01). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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