Molecular serotyping of Haemophilus parasuis isolated from diseased pigs and the relationship between serovars and pathological patterns in Taiwan

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Microbiology

Main article text

 

Introduction

Materials and Methods

Bacterial isolate collection and identification

Molecular serotyping mPCR

Sequencing and analysis of unexpected PCR-amplified products

Pathological examination

Detection of porcine reproductive and respiratory syndrome virus

Statistical analysis

Results

H. parasuis isolates, origins and pathological lesion patterns

Serovar distribution by molecular serotyping assay

Identification of serovar-specific amplicons

Serovar distribution based on molecular serotyping assay and sequencing

Relationship between pathological lesion patterns and serovars

Discussion

Conclusions

Supplemental Information

Gross meningeal lesion in H. parasuis infected pigs.

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Histopathological suppurative meningitis lesion in H. parasuis infected pigs.

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Gross pleural and peritoneal lesions in H. parasuis infected pigs.

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Gross epicardial lesion in H. parasuis infected pigs.

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Gross joint synovial cavity lesion in H. parasuis infected pigs.

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Histopathological fibrinous serositis lesion in H. parasuis infected pigs.

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Gross lung lesion in H. parasuis infected pigs.

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Histopathological pulmonary lesion in H. parasuis infected pigs.

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Histopathological fibrous serositis lesion in H. parasuis infected pigs.

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Histopathological meningitis lesion in H. parasuis infected pigs.

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Band patterns of molecular serotyping mPCR for Haemophilus parasuis.

Lane M: 50 bp DNA Ladder, lane S5: serovar 5 or 12, lane G2: molecular serotyping non-typable group 2, lane S4: serovar 4, lane S9: serovar 9, lane G1: molecular serotyping non-typable group 1, lane NC: negative control.

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Band patterns of molecular serotyping mPCR for Haemophilus parasuis.

Lane M: 50 bp DNA Ladder, lane S4: serovar 4, lane S5: serovar 5 or 12, lane G2: molecular serotyping non-typable group 2, lane S14: serovar 14, lane G1: molecular serotyping non-typable group 1, lane NC: negative control.

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Band patterns of molecular serotyping mPCR for Haemophilus parasuis.

Lane M: 50 bp DNA Ladder, lane S5: serovar 5 or 12, lane S4: serovar 4, lane G1: molecular serotyping non-typable group 1, lane G2: molecular serotyping non-typable group 2, lane NC: negative control. Histopathological bronchopneumonia lesion in H. parasuis infected pigs.

DOI: 10.7717/peerj.6017/supp-13

Band patterns of molecular serotyping mPCR for Haemophilus parasuis.

Lane M: 50 bp DNA Ladder, lane G1: molecular serotyping non-typable group 1, lane S4: 608 serovar 4, lane S5: serovar 5 or 12, lane S14: serovar 14, lane G4: molecular serotyping non- 609 typable group 4, lane G3: molecular serotyping non-typable group 3, lane NC: negative control.

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Band patterns of molecular serotyping mPCR for Haemophilus parasuis.

Lane M: 50 bp DNA Ladder RTU (GeneDireX), lane G2: molecular serotyping non-typable group 2, lane S4: serovar 4, lane S5: serovar 5 or 12, lane G1: molecular serotyping non-typable group 1, lane G3: molecular serotyping the non-typable group 3, lane NC: negative control.

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Serovar distribution of Haemophilus parasuis in different countries or regions by different serotyping assays.

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Cases distinguished by complete pathological examination or not.

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Relationship between pathological diagnoses and serovers.

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Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Wei-Hao Lin conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper.

Hsing-Chun Shih performed the experiments.

Chuen-Fu Lin contributed reagents/materials/analysis tools.

Cheng-Yao Yang contributed reagents/materials/analysis tools.

Yung-Fu Chang contributed reagents/materials/analysis tools.

Chao-Nan Lin conceived and designed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Ming-Tang Chiou conceived and designed the experiments, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The study did not involve any animal experiment. The Institutional Animal Care and Use Committee (IACUC) of National Pingtung University of Science and Technology did not deem it necessary for this research group to obtain formal approval to conduct this study.

Data Availability

The following information was supplied regarding data availability:

The raw data are provided in Table S2.

Funding

This study was supported by the Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Taiwan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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