The pht4;1-3 mutant line contains a loss of function allele in the Fatty Acid Desaturase 7 gene caused by a remnant inactivated selection marker—a cautionary tale

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Introduction

Materials and methods

Plant material, growth conditions and genotyping

Extraction and quantification of lipids

Results and Discussion

Conclusions

Supplemental Information

DNA sequence of insert located in FAD7 in the pht4;1- 3 mutant line

Letters marked in blue indicate IAAH stop codon and letters in yellow indicate FAD7 genomic sequence.

Genomic sequence of FAD7 from wild type Col-0 (TAIR12, http://www.arabidopsis.org). Start and stop codon are highlighted in blue. Capital letters indicate exon and lower case letters indicate introns. Yellow letters indicate sequence identified in the pht4;1-3 mutant (see sequence above).

DOI: 10.7717/peerj.4134/supp-1

Raw data for Fig. 1A and Table 1

Figure 1A. Galactolipid species composition in wt and pht. A total lipid extract from the indicated lines was analysed by LC-MS/MS and the composition of MGDG and DGDG was determined.

Table 1. Fatty acid composition of leaf tissue. Fatty acid methykl esters were obtained by direct alkaline transmethylation of leaf tissue and analyzed by GC-MS, fatty acids quantified as mol%

DOI: 10.7717/peerj.4134/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Anders K. Nilsson conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Mats X. Andersson conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Availability

The following information was supplied regarding data availability:

The raw data has been provided as a Supplemental File.

Funding

This work was supported by the Carl Tryggers foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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