Identification and analysis of CYP450 genes from transcriptome of Lonicera japonica and expression analysis of chlorogenic acid biosynthesis related CYP450s

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Introduction

Materials and Methods

Identification of CYP450 genes in L. japonica

Classification and characterization of L. japonica CYP450 genes

Phylogenetic analysis of predicted CYP450 genes

Gene ontology and KEGG pathway analysis

Extraction and quantification of CGA

RNA extraction and qRT-PCR

Results

Identification and classification of CYP450 genes in L. japonica

Phylogenetic analysis of predicted CYP450s in L. japonica

Conserved motifs analysis of L. japonica CYP450s

Gene ontology classification of L. japonica CYP450s

KEGG pathway analysis of L. japonica CYP450s

CYP450s involved in CGA biosynthesis

Discussion

Conclusions

Supplemental Information

Supplemental Table 1

Primers used for qRT-PCR

DOI: 10.7717/peerj.3781/supp-1

Sequences of LjC4H3 and LjC3H2

DOI: 10.7717/peerj.3781/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Xiwu Qi performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Xu Yu and Daohua Xu performed the experiments.

Hailing Fang analyzed the data.

Ke Dong contributed reagents/materials/analysis tools.

Weilin Li and Chengyuan Liang conceived and designed the experiments, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The LjC4H3 and LjC3H2 sequences described here are accessible via GenBank accession numbers KX845341 and KX845342.

Funding

The work was supported by the National Natural Science Foundation of China (31500249), Six Talent Peaks Project in Jiangsu Province (2015-NY-032), the Natural Science Foundation of Jiangsu Province (BK20161381), and the Foundation of Jiangsu Key Laboratory for the Research and Uti1ization of Plant Resources (SQ201401). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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