Coronatine inhibits stomatal closure and delays hypersensitive response cell death induced by nonhost bacterial pathogens

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PeerJ

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Introduction

Materials and Methods

Plant and pathogen materials

Bacterial pathogen inoculation

Stomata assay

Inhibition of nonhost HR cell death by COR and bacterial growth determination

RNA isolation and quantitative real-time PCR

Results and Discussion

Pseudomonas syringae pv. tabaci inoculation on tomato induces rapid closure of stomata and nonhost HR

Coronatine inhibits stomatal closure induced by nonhost pathogen, P. syringae pv. tabaci

Coronatine delays nonhost HR cell death induced by nonhost pathogen P. syringae pv. tabaci

Coronatine represses the expression of SA-related defense genes during nonhost resistance responses in tomato

Conclusion

Supplemental Information

Supplemental Figure S1

Development of disease symptoms by host pathogen P. syringae pv. tomato DC3000 in tomato. (A) Tomato leaves were spray-inoculated with P. syringae pv. tomato DC3000 (5 × 103 CFU/ml) and P. syringae pv. tabaci (5 × 103 CFU/ml), and imaged five days after inoculation. (B) The nonhost HR cell death by P. syringae pv. tabaci in tomato. Tomato leaves were syringe-infiltrated with host pathogen P. syringae pv. tomato DC3000 (6 × 102 CFU/ml) or nonhost pathogen P. syringae pv. tabaci (6 × 102 CFU/ml). The nonhost HR cell death was observed 48 h after infiltration.

DOI: 10.7717/peerj.34/supp-1

Supplemental Table 1

DOI: 10.7717/peerj.34/supp-2

Additional Information and Declarations

Competing Interests

Kirankumar S. Mysore is an Academic Editor for PeerJ. The other authors do not have any competing interests.

Author Contributions

Seonghee Lee conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper.

Yasuhiro Ishiga and Kristen Clermont performed the experiments.

Kirankumar S. Mysore conceived and designed the experiments, analyzed the data, wrote the paper.

Funding

This work was funded by the Samuel Roberts Noble Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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