Transient fusion and selective secretion of vesicle proteins in Phytophthora nicotianae zoospores

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2046 days ago
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
2046 days ago
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
2046 days ago
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
2047 days ago
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
RT @egilro: We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicle…
2047 days ago
We are new custodians of 6 Phytopthora antibodies from the Hardham group raised against proteins from: large peripheral vesicles, mastigonemes and cyst walls. https://t.co/vY1NNW2WZh … https://t.co/WnC5VAyU0O https://t.co/PcnBHTomS1 … Please contact us if interested in using. https://t.co/UM8qjjAaW3
PeerJ

Main article text

 

Introduction

Materials and Methods

P. nicotianae isolate and culture conditions

Expression of recombinant PnCcp and production of polyclonal antibodies

Immunoblot analysis

Preparation of material for immunolabelling

Immunofluorescence labelling

Immunogold labelling

Microscopy

Enzyme-linked immunosorbent assay

Dynamin inhibitor experiments

Gene expression analysis

Identification of dynamin homologs in P. nicotianae

Results

Selective secretion of PnCcp from P. nicotianae zoospore large peripheral vesicles

Verification of the specificity of the PnCcpCpep antibody

Timing of secretion of PnCcp

Synthesis of PnCcp and PnLpv proteins

Timing of expression of PnCcp and PnLpv genes

Compartmentalization of PnCcp in large peripheral vesicles

Role of dynamin in selective secretion of PnCcp

Discussion

A transient fusion mechanism underlies selective secretion of PnCcp

Factors underlying selective secretion of PnCcp

Concluding remarks

Additional Information and Declarations

Competing Interests

Adrienne Hardham is an Academic Editor for PeerJ.

Author Contributions

Weiwei Zhang, Leila M. Blackman and Adrienne R. Hardham conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper.

Funding

The research was funded by Australian Research Council grants to ARH and a Chinese Scholarship Council scholarship to WZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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