Genetic and demographic recovery of an isolated population of brown bear Ursus arctos L., 1758

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Introduction

Materials and Methods

Population monitoring

Sampling collection and DNA extraction

Microsatellite loci amplification

Genetic diversity analyses

Genetic and spatial variation between subpopulations

Relatedness analyses

Estimate of effective population size

Results

Population monitoring

Microsatellite dataset preparation and sex determination

Genetic diversity

Population structure and assignment of individuals

Relatedness analyses

Estimate of effective population size (Ne)

Discussion

Supplemental Information

Unique genotypes for the Cantabrian brown bear samples.

Unique genotypes obtained for Cantabrian brown bear individuals collected at the eastern and western subpopulations respectively. The name of the microsatellite loci used is also indicated.

DOI: 10.7717/peerj.1928/supp-1

Summary of sample collection and genetic analyses performed on brown bear (Ursus arctos) from the Cantabric Mountain.

DOI: 10.7717/peerj.1928/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Elena G. Gonzalez conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Juan C. Blanco conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Fernando Ballesteros conceived and designed the experiments, analyzed the data, prepared figures and/or tables reviewed drafts of the paper.

Lourdes Alcaraz performed the experiments.

Guillermo Palomero conceived and designed the experiments, coordinate the sample collection and obtained funding.

Ignacio Doadrio conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The performed research did not involve any experiments with animals and complies with international ethical guidelines.

The permission established with the autonomous region of Castile and Leon to collect the samples and monitor the population is available upon the request of the editor and reviewers.

Data Deposition

The following information was supplied regarding data availability:

Unique genotypes obtained for the Cantabrian brown bear can be found in the Supplementary Information.

Funding

This research was funded by Enel GreenPower Spain. The General Directorates of Nature Conservation of the autonomous regions of Castile and Leon, and Asturias provided logistic support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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