Acptp2,3 participates in the regulation of spore production, stress response, and pigments synthesis in Aspergillus cirstatus

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials & Methods

Experimental materials

Experimental methods

Analysis of Actp2,3 sequence

Screening and identification of the ΔAcptp2,3 and ΔAcptp2,3-C strain

Morphological observation

Statistical analysis

Real time-PCR detection

Results and Analysis

Screening and identification of the ΔAcptp2,3 and ΔAcptp2,3-C strain

Acptp2,3 promoted the production of ascospores and conidia in A. cristatus

Deleting Acptp2,3 gene effects the response of A. cristatus to high osmotic stress

Role of Acptp2,3 under oxidative stress

Acptp2,3 was involved in the response of Congo red and sodium dodecyl sulfate in A. cristatus

Deleting Acptp2,3 caused colonies to deepen in color

RT-qPCR analysis of related gene expression

Discussion

Conclusions

Supplemental Information

Data for Figures 3-10

DOI: 10.7717/peerj.17946/supp-2

Table S1, Figure S1, Figure S6

Primer information in this study.

DOI: 10.7717/peerj.17946/supp-3

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Lei Shao conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Zuoyi Liu conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Yumei Tan performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The data is available at NCBI: JXNT01000002.1.

Funding

This research was funded by the Guiyang Healthcare Vocational University Doctoral Fund (No. Guiyang Healthcare Vocational University K2024-8), the Central Government Guidance for Local Science and Technology Development Projects for Guizhou Province (No. [2023]027), the Youth Foundation of Guizhou Academy of Agricultural Sciences (No. [2023]05), and the Science and Technology Foundation of Guizhou Province (No. qiankehejichu-ZK [2022] general482). There was no additional external funding received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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