Phylogenetic synthesis of morphological and molecular data reveals insights on the classification of diogenid hermit crabs (Crustacea: Decapoda: Anomura)

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Zoological Science

Main article text

 

Introduction

Materials and Methods

Taxon sampling and data collection

DNA extraction, PCR amplification and sequencing

Phylogenetic analyses

Ancestral character state reconstruction

Results

Discussion

Discordance between mtDNA and nDNA trees

Paraphyletic diogenidae with coenobitidae nested

Generic-level relationships within diogenidae

Conclusions

Supplemental Information

The data matrix of morphological characters.

DOI: 10.7717/peerj.17922/supp-1

Primer sets used in PCR amplification of the four genes.

DOI: 10.7717/peerj.17922/supp-2

Best partitioning scheme and best-fit substitution model selected by PartitionFinder.

DOI: 10.7717/peerj.17922/supp-3

Previous molecular hypotheses of phylogenetic relationships among diogenid genera (Calcinus, Clibanarius and Dardanus) and coenobitid genera (Coenobita and Birgus).

DOI: 10.7717/peerj.17922/supp-4

Phylogenetic relationships among diogenid and coenobitid genera resolved by Bayesian inference analysis based on combined molecular and morphological datasets.

Nodal support values represent Bayesian posterior probabilities (Pp). Two Pagurus species from the family Paguridae were used to root the phylogenetic tree.

DOI: 10.7717/peerj.17922/supp-5

Phylogenetic relationships among diogenid and coenobitid genera based on combined mitochondrial DNA (A) and combined nuclear DNA (B) sequences.

Nodal support values represent maximum likelihood bootstrap (bs)/Bayesian posterior probabilities (Pp). Only bootstrap support values above 50% are shown.

DOI: 10.7717/peerj.17922/supp-6

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jiao Cheng conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Wenjie Li performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Yanrong Wang analyzed the data, prepared figures and/or tables, and approved the final draft.

Zhongli Sha conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

All sequences obtained in the present study are available at GenBank: MK610008MK610036; MK610039MK610041; MH508045MH508054; MH508056MH508087; MK747765MK747780; MK747782MK747788; MK747790MK747812; MW411962; MW411963; MW413814; MW413815.

Funding

This work was supported by the National Key R&D Program of China (No. 2022YFC3102403), the National Science Foundation for Distinguished Young Scholars (No. 42025603), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDB42000000), and the Qingdao New Energy Shandong Laboratory Open Project (No. QNESL OP202306). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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