In vitro cytoprotective and in vivo anti-oral mucositis effects of melatonin and its derivatives

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Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

In vitro studies on HaCaT cells

Cytoprotective effect in H2O2-damaged cells

where, cell death being estimated from 100–%cell viability. The H2O2 group was considered as 0% cell protective.

Cellular antioxidant activity

Chemoprotective effect against 5-fluorouracil induced cells damage

In vivo 5-FU-induced oral mucositis in mice

Statistical analysis

Results

Discussion

Conclusions

Supplemental Information

Author checklist.

DOI: 10.7717/peerj.17608/supp-1

Raw data of Chemoprotective.

DOI: 10.7717/peerj.17608/supp-2

Weight and Food consumption.

DOI: 10.7717/peerj.17608/supp-3

Supplementary data of Cytotoxic MLT EBM BBM.

DOI: 10.7717/peerj.17608/supp-4

Raw data of cytotoxicity.

DOI: 10.7717/peerj.17608/supp-5

Raw data of H2O2- treated for cytotoxicity.

DOI: 10.7717/peerj.17608/supp-6

Raw data of ROS.

DOI: 10.7717/peerj.17608/supp-7

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Pramote Mahakunakorn conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Pimpichaya Sangchart performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Panyada Panyatip performed the experiments, prepared figures and/or tables, and approved the final draft.

Juthamat Ratha performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Teerasak Damrongrungruang conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Aroonsri Priprem conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Ploenthip Puthongking conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Northeast Laboratory Animal Center, Khon Kaen University, Thailand.

Data Availability

The following information was supplied regarding data availability:

The raw data are available in the Supplemental Files.

Funding

The research has received funding support from Khon Kaen University-National Research Council of Thailand (KKU-NRCT), and the National Science, Research and Innovation Fund (NSRF), Thailand via the Fundamental Fund of Khon Kaen University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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