Curcumin improves atrial fibrillation susceptibility by regulating tsRNA expression in aging mouse atrium

View article
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Methods and materials

Animals

Rapid transjugular atrial pacing

PANDORA-seq

Small RNA library construction and sequencing

Small RNA annotation

Target mRNA prediction and correlation analysis of differential tsRNA

Cell culture and mock transfection

Tissue and cell senescence β-gal staining

ROS detection

Validation by qRT-PCR

Statistical analysis

Results

Curcumin reduced susceptibility to AF by attenuating atrial aging and inflammation in aged mice

Exemplary annotation and profiling of PANDORA-seq datasets generated by SPORTS1.1

General characteristics of tsRNAs expression

TsRNA expression difference and target gene prediction

qRT-PCR verification of differential tsRNA and target genes

Mature-mt_tRNA-Val-TAC_CCA_end regulated the celluar senecence and the levels of ROS and inflammatory factors

Discussion

conclusion

Supplemental Information

TsRNA expression profiles between the 18 month and 18 month+Cur group.

DOI: 10.7717/peerj.17495/supp-1

Target genes of differential tsRNAs.

DOI: 10.7717/peerj.17495/supp-2

Differentially expressed tsRNAs between 5 month and 18 month.

DOI: 10.7717/peerj.17495/supp-3

Electrophysiology Data.

Rapid atrial pacing was performed through the electrophysiological characteristics of mice

DOI: 10.7717/peerj.17495/supp-4

qPCR data for Figure 1.

DOI: 10.7717/peerj.17495/supp-5

qPCR data for Figure 5.

DOI: 10.7717/peerj.17495/supp-6

qPCR data for Figure 6.

DOI: 10.7717/peerj.17495/supp-7

qPCR data for Figure 7.

DOI: 10.7717/peerj.17495/supp-8

A completed full ARRIVE 2.0 checklist.

DOI: 10.7717/peerj.17495/supp-9

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Xueshan Luo conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Panyue Liu conceived and designed the experiments, performed the experiments, prepared figures and/or tables, and approved the final draft.

Xingdong Ye performed the experiments, prepared figures and/or tables, and approved the final draft.

Jintao He performed the experiments, prepared figures and/or tables, and approved the final draft.

Yingyu Lai performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Yidong Lv analyzed the data, prepared figures and/or tables, and approved the final draft.

Xiongbin Wu conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Yang Liu analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Qianhuan Zhang analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Hui Yang performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Wei Wei performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Chunyu Deng analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Sujuan Kuang performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Shulin Wu analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Yumei Xue analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Fang Rao conceived and designed the experiments, analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

All procedures and protocols in this study have been approved by the animal experiment ethics committee of Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences (ethics number GDREC2016128A).

Data Availability

The following information was supplied regarding data availability:

The data is available in the Supplemental File and at NCBI GEO: GSE217761.

Funding

This work was supported by the High-level Hospital Construction Plan (Nos. DFJH201808 and DFJH201925), the Guangdong special funds for science and technology innovation strategy, China (Stability support for scientific research institutions affiliated to Guangdong Province-GDCI 2021) and the Science and Technology Program of Guangdong Province (No. 2019B020230004). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

1 Citation 1,387 Views 60 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more