PCMT1 knockdown attenuates malignant properties by globally regulating transcriptome profiles in triple-negative breast cancer cells

View article
Biochemistry, Biophysics and Molecular Biology

Main article text

 

Introduction

Materials and Methods

Lentivirus

Culture and shRNA transfection of cells

The proliferation assay

Flow cytometric analysis of cell apoptosis

Cell invasion assay

Cell migration assay

RNA extraction and sequencing

RNA-seq quality filtering and differentially expressed genes analysis

AS analysis

Reverse transcription and quantitative polymerase chain reaction

Functional enrichment analysis

Results

PCMT1 knockdown inhibits the proliferation of MDA-MB-231 cells

PCMT1 knockdown inhibits the migration and invasion of MDA-MB-231 cells

PCMT1 regulates the global gene expression profile in MDA-MB-231 cells

PCMT1 regulates the expression level of RBPs

PCMT1 regulates the AS pattern of transcripts in MDA-MB-231 cells

Discussion

Supplemental Information

Supplemental Figures

DOI: 10.7717/peerj.16006/supp-1

Primer sequences for RT-qPCR experiments

DOI: 10.7717/peerj.16006/supp-2

Raw data of cellular phenotype results

DOI: 10.7717/peerj.16006/supp-3

Raw data of DEGs RT-qPCR results

DOI: 10.7717/peerj.16006/supp-4

Raw data of RASEs RT-qPCR results

DOI: 10.7717/peerj.16006/supp-5

Enriched GO BP pathways for RASGs

DOI: 10.7717/peerj.16006/supp-6

Additional Information and Declarations

Competing Interests

Xinyu Hu and Dong Chen are employed by Wuhan Ruixing Biotechnology Co., Ltd.. The authors declare there are no competing interests.

Author Contributions

Aili Saiding performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Dilinuer Maimaitiyiming performed the experiments, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Minglan Chen performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Futian Yan performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Dong Chen analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Xinyu Hu analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Ping Shi conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The data are available at NCBI GEO: GSE225762.

Funding

The authors received no funding for this work.

3 Citations 1,176 Views 86 Downloads

Your institution may have Open Access funds available for qualifying authors. See if you qualify

Publish for free

Comment on Articles or Preprints and we'll waive your author fee
Learn more

Five new journals in Chemistry

Free to publish • Peer-reviewed • From PeerJ
Find out more