Chemically mediated rheotaxis of endangered tri-spine horseshoe crab: potential dispersing mechanism to vegetated nursery habitats along the coast

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Chemically mediated rheotaxis of endangered tri-spine horseshoe crab: potential dispersing mechanism to vegetated nursery habitats along the coast https://t.co/vBkQPIsXkn @thePeerJ https://t.co/TvPUaFXNKk
Aquatic Biology

Main article text

 

Introduction

Materials & Methods

Larval and juvenile horseshoe crab rearing

Experimental setup and conditioned water preparation

Orientation and movement behaviors toward chemical cues

Statistical analysis

Results

Discussion

Conclusions

Supplemental Information

The orientation and movement behaviors of juvenile horseshoe crabs in responses to various habitat vegetation cues at different exposure concentrations

DOI: 10.7717/peerj.14465/supp-1

Results of Student’s t tests (d.f.= 4) showing the effect of different inflow position of test waters (conditioned/control seawater), i.e., whether on the left or the right arm of the Y-maze chamber, on the behavioral responses of juvenile [i]T. tr

Since the orientation data are not normally distributed, non-parametric Mann-Whitney U tests are adopted for the analyses. Conc., Concentration.

DOI: 10.7717/peerj.14465/supp-2

Preliminary results on microalgae compositions and densities (105 cells l−1) on the sediment surface of intertidal flats within Asian horseshoe crab nursery habitats along the northern Beibu Gulf shores, China

One sample was collected per site.

DOI: 10.7717/peerj.14465/supp-3

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Kit Yue Kwan conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Xin Yang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Chun-Chieh Wang analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Yang Kuang conceived and designed the experiments, performed the experiments, prepared figures and/or tables, and approved the final draft.

Yulong Wen performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Kian Ann Tan analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Peng Xu analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Wenquan Zhen analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Xueping Wang conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Junhua Zhu conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Xing Huang conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements are available in the Supplementary File.

Funding

This work was funded by the National Natural Science Foundation of China (32060129), the Beibu Gulf Ocean Development Research Centre under Key Research Base of Humanities and Social Sciences in Guangxi Universities, Marine Science Program for Guangxi First-Class Discipline, Beibu Gulf University (DRA002, TRA001), and the Guangxi Recruitment Program of 100 Global Experts. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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