The antioxidant system response to drought-stressed Diospyros lotus treated with exogenous melatonin

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Plant Biology

Main article text

 

Introduction

Materials & Methods

Plant material and experiment design

Determination of Chlorophyll fluorescence

Determination of superoxide, H2O2 and Malonaldehyde

Analysis of antioxidative enzyme activities

Determination of nitric oxide content and nitrate reductase activity

qRT-PCR analysis

Statistical analysis

Results

Effects of exogenous melatonin on O2•−, H2O2, and malondialdehyde content

Effects of exogenous melatonin on the maximum efficiency of photosystem II photochemistry (Fv/Fm)

Effects of exogenous melatonin on ROS production and scavenging enzymes

Effects of exogenous melatonin on NO accumulation

Effects of exogenous melatonin on key genes of ROS metabolism

Discussion

Conclusions

Supplemental Information

Composition of Hoagland nutrient solution

DOI: 10.7717/peerj.13936/supp-1

Primer sequences (5′→ 3′) for qRT-PCR

DOI: 10.7717/peerj.13936/supp-2

Additional Information and Declarations

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Peng Zhang conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Yi Hu performed the experiments, analyzed the data, prepared figures and/or tables, and approved the final draft.

Ruijin Zhou conceived and designed the experiments, performed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Xiaona Zhang analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Huiling Hu conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Dongmei Lang conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw data is available at Zenodo: Zhangpeng. (2022). The antioxidant system response to drought-stressed Diospyros lotus treated with exogenous melatonin [Data set]. Zenodo. https://doi.org/10.5281/zenodo.6526608.

Funding

This work was funded by the Scientific and Technological Research Project of Henan Province (No. 202102110051 and 202102110054), and the Scientific Research Starting Foundation of Henan Institute of Science and Technology (No. 103010620001/003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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