Genetic diversity of enterotoxigenic Bacillus cereus strains in coriander in southwestern Mexico

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Microbiology

Main article text

 

Introduction

Materials and Methods

Coriander samples

Microbiological analysis

Molecular identification and toxin gene profiling of B. cereus isolates

Biofilm production

Determination of genes related to biofilm production in B. cereus

Enzymatic profile

Analysis of presumed psychotropic properties

Determination of sporulation rates

Genetic diversity of B. cereus strains

Statistical analysis

Results

Discussion

Conclusions

  • The prevalence of B. cereus in vegetables by molecular identification of the gyrB gene was 20%

  • The most common enterotoxin gene was nhe [76.4%, (13/17)], followed by cytK [82.3%, (14/17)]

  • In the case of genes related to biofilms, the frequency was low for sipW [5.8%, (1/17)] and tasA [11.7%, (2/17)]

  • The sporulation rates could be responsible for the presence of B. cereus in vegetables due to the resist characteristic of the spores previously reported.

  • Further analyses are necessary for identification and molecular characterization of other strains of B. cereus in vegetables for human consumption.

Supplemental Information

Raw data of sporulation rates and biofilm.

DOI: 10.7717/peerj.13667/supp-1

Uncropped gels.

The GTG figure was taken in a different capture system

DOI: 10.7717/peerj.13667/supp-2

Additional Information and Declarations

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Daniel Alexander Castulo-Arcos performed the experiments, prepared figures and/or tables, and approved the final draft.

Roberto Adame-Gómez performed the experiments, prepared figures and/or tables, and approved the final draft.

Natividad Castro-Alarcón conceived and designed the experiments, prepared figures and/or tables, and approved the final draft.

Aketzalli Galán-Luciano performed the experiments, authored or reviewed drafts of the article, discussion, and approved the final draft.

María Cristina Santiago Dionisio conceived and designed the experiments, authored or reviewed drafts of the article, and approved the final draft.

Marco A. Leyva-Vázquez analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Jose-Humberto Perez-Olais analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Jeiry Toribio-Jiménez analyzed the data, authored or reviewed drafts of the article, and approved the final draft.

Arturo Ramirez-Peralta conceived and designed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the article, and approved the final draft.

Data Availability

The following information was supplied regarding data availability:

The raw measurements of sporulation rates and biofilm are available in the Supplemental Files.

Funding

Daniel Alexander Castulo-Arcos received a fellowship from the National Science and Technology Council as support for this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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